栀子黄色素纯化工艺的优化  

Optimization of purification technology of gardenia yellow pigment

作　　者：张徐[1] 方吉雷 葛青[1,2,3] ZHANG Xu FANG Jilei GE Qing(School of Biological and Chemical Engineering,Zhejiang University of Science and Technology, Hangzhou 310023, China Zheiiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Produce, Hangzhou 310023, China Zhejiang Collaborative Innovation Center of Chemical and Biological Manufacturing for Agricultural Biological Resources, Hangzhou 310023, China)

机构地区：[1]浙江科技学院生物与化学工程学院,杭州310023 [2]浙江省农产品化学与生物加工技术重点实验室,杭州310023 [3]浙江省农业生物资源生化制造协同创新中心,杭州310023

出　　处：《浙江科技学院学报》2016年第6期456-461,共6页Journal of Zhejiang University of Science and Technology

基　　金："十二五"农村领域国家科技计划课题(2011BAD23B02)

摘　　要：为了提高栀子黄色素品质和提升产品附加值,采用超声辅助有机溶液热回流萃取法制备栀子黄色素,采用大孔树脂分离法分离纯化栀子黄色素。实验结果表明,栀子黄色素的最佳提取条件为60%体积分数的乙醇溶液,1∶10料液比,60℃超声反应30min,提取2次;栀子黄色素的最佳纯化条件为用25%体积分数的乙醇溶液洗脱大孔树脂吸附的杂质、栀子苷、绿原酸,用80%体积分数的乙醇溶液分离栀子黄色素,经纯化得到的栀子黄色素色价大于320,OD值小于0.2。To improve the quality of the gardenia yellow pigment and improve the product added value, organic solvent extraction with ultrasonic-assisted was used to obtain gardenia yellow pigment, and macroporous resin adsorption method was used to purify gardenia yellow pigment. The results show that the optimal conditions of extraction are that solid to liquid ratio 1 L 10, the concentration of ethanol 60% , extraction temperature of 70℃,extraction time of 30 min, extraction twice. And the optimal conditions of purification are using 25 % ethanol eluting macroporous resin adsorption solution to elute impurities, chlorogenic acid and gardenoside; using 80 % ethanol solution to separate gardenia yellow pigment. As a result, the color scale of gardenia yellow pigment is more than 320, OD value is less than 0.2.

关 键 词：栀子黄色素 纯化工艺 大孔树脂 

分 类 号：TS264.4[轻工技术与工程—发酵工程] R284.2[轻工技术与工程—食品科学与工程]