一株鲢鱼肠道中嗜水气单胞菌拮抗菌株的筛选、鉴定及特性  

Screening,Identification and Properties of One Antagonistic Strain Against Aeromonas hydrophila from Hypophthalmichthys molitrix Intestinal Tract

作　　者：洪军[1] 白玉珍[1] 马肖梦 韩莎[1] 孔丽[1] 

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036

出　　处：《食品与生物技术学报》2016年第12期1285-1291,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31540060);2014年度河南城建学院大学生科技创新基金项目;河南城建学院博士启动基金项目

摘　　要：从鲢鱼肠道中筛选嗜水气单胞菌的拮抗菌株,并对生长培养条件进行研究,以期为鲢鱼养殖过程中嗜水气单胞菌病的生物防治提供理论依据。通过对鲢鱼肠道中嗜水气单胞菌拮抗菌株的分离、筛选,并对其中拮抗作用较稳定的菌株进行生理生化、分子鉴定以及生长培养特性优化。经过多次筛选得到了15株拮抗效果较好的目标菌株,其中拮抗作用较稳定的S4为气单胞菌属,是偏碱性革兰氏阴性短杆菌;通过单因素和正交实验表明,S4最佳生长培养条件为1.5 g/d L淀粉,1.5 g/d L牛肉膏,1 g/d L氯化钙,32℃,pH 8.5,接种体积分数3%。研究结果为嗜水气单胞菌的防治及拮抗菌S4的应用提供理论依据。In order to provide the theory basis for prevention and treatment the disease caused by Aeromonas hydrophila in the process of Hypophthalmichthys molitrix breeding,the strains which have the strong and stable abilities against A. hydrophila from H. molitrix intestinal tract were screened and their growth and culture conditions were studied. Antagonistic strains against A. hydrophila were separated and screened from H. molitrix intestinal tract. Then,the physiological and biochemical,molecular identification and the optimization of growth conditions of the stable antagonistic bacterium were determined. After many times of screening,15 antagonistic strains were got. Among them,the more stable antagonistic bacterium is number S4,which was an alkaline Gram-negative bacillus brevis bacteria and belonged to Aeromonas genus. Orthogonal test indicated that the optimal growth conditions were 1.5 g/dL starch,1.5 g/dL beef extract,1 g/dL CaCL2,32 ℃,pH 8.5 and inoculation quantity 3%. The results provide the theoretical basis for the prevention of A. hydrophila and the application of antagonistic bacterium S4.

关 键 词：嗜水气单胞菌 鲢鱼 拮抗作用 菌株鉴定 S4 生长培养条件 

分 类 号：S943[农业科学—水产养殖]