谷氨酰胺转胺酶诱导交联发酵乳蛋白的体内抗氧化功效  

Anti-Oxidative Activity of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein in vivo

作　　者：吕建敏[1] 袁海娜[2] 

机构地区：[1]浙江中医药大学动物实验研究中心,浙江杭州310053 [2]浙江科技学院生物与生化工程学院/轻工学院,浙江杭州310023

出　　处：《食品与生物技术学报》2016年第12期1330-1335,共6页Journal of Food Science and Biotechnology

基　　金：浙江省自然科学基金项目(LY14C200007)

摘　　要：为了研究微生物谷氨酰胺转胺酶诱导交联发酵乳蛋白(Microbial Transglutaminase CrossLinked Fermentation Milk Protein,m TG-FPM)对D-半乳糖(D-gal)衰老模型小鼠的抗氧化活性的影响,试验设正常组、D-gal模型组、D-gal+0Um TG-FPM组、D-gal+1Um TG-FPM组、D-gal+3Um TG-FPM组和D-gal+VE阳性对照组共6组,每组10只C57BL/J小鼠。采用皮下连续注射D-Gal方式建立衰老小鼠模型。造模同时,FPM各组每天灌服1.5 g/kg m TG-FPM/FPM,D-gal+VE阳性对照组每天灌服100 mg/kg的VE,模型对照和正常组每天灌服蒸馏水0.2 m L/10 g。连续灌胃8 w后,测定各组小鼠肝肾组织和血清中的相关抗氧化指标(CAT、SOD、GSH-Px MDA)。结果表明:D-Gal可显著降低衰老模型小鼠肝脏和血清中CAT及GSH-Px活性(P<0.01,P<0.05);显著降低肾脏和血清中的SOD活力(P<0.01,P<0.05),显著升高MDA含量(P<0.01)。与模型组和0Um TG组相比,3Um TG组的肝脏CAT,1Um TG组血清CAT活性和3U m TG组肝脏GSH-Px活性显著升高(比0Um TG组分别升高11.14%、35.57%和22.36%,P<0.01,P<0.05);而3Um TG组肾脏中的MDA含量显著降低(比0Um TG组降低26.41%,P<0.05)。因此,m TG诱导交联处理可在一定程度上改善FPM的体内抗氧化活性。The study was carried out to investigate the anti-oxidative effect of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein（mTG-FPM） in aging mice induced by D-galactose（D-gal）. Sixty C57BL/6J female mice（12 w old）were divided into 6 groups including the control group,D-gal model group,D-gal-induced mice treated with FPM cross-linked mTG by 0 U,1 U and 3 U/g protein（as D-gal＋0 UmTG-FMP,D-gal＋1UmTG-FMP and D-gal＋3 UmTG-FMP groups）,at 1.5 g/kg bw via ig,and D-gal-induced mouse treated with vitamin E as the positive group at 100 mg/kg via ig,respectively. After continuous administration for 8 weeks,the anti-oxidative parameters（CAT,SOD,MDA GSH-Px） in liver,kidney tissues,and serum of mice in each group were determined. The results showed that mice induced by D-gal had lower CAT and GSH-Px activity in liver and serum,lower SOD activity of kidney and serum,and higher MDA content of kidney and serum,when compared with the normal mice（P 〈 0.01,P 〈 0.05）. Compared to the model and untreated FPM groups,FPM treated with mTG had higher CAT activities in liver tissue（3 U mTG treatment,P 〈 0.01） and in serum（1 U mTG treatment,P 〈 0.05） up to 11.14% and 35.57% respectively,whereas the content of MDA in kidney tissue（P 〈 0.01） in FPM treated with mTG was reduced 26.41%. The activity of GSH-Px in liver tissue was also sharply higher（22.36% increase） in D-gal＋3 U mTG-FPM group than that of in FPM group untreated with mTG（P 〈 0.01）. It was concluded that the mTG Cross-linked effect in FPM can improve the anti-oxidative ability of aging mice induced by D-gal.

关 键 词：微生物谷氨酰胺转胺酶 发酵乳蛋白 体内 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]