HPLC同步测定多种合成色素含量检测结果的不确定度评定  

Uncertainty Evaluation on Measurement Results for Simultaneous Determination on Synthetic Pigments by HPLC

作　　者：伍雅婷[1] 郭怡阳[2] 黄常刚[1] 刘俊玲[1] 吴晓旻[1] 孙言凤[1] 李静娜[1] WU Ya-ting GUO Yi-yang HUANG Chang-gang LIU WU Xiao-min SUN Yan-feng LI Jing-na(Wuhan Center for Disease Control and Prevention, Wuhan 430015, China Wuhan Women and Children＇s Health Care Center, Wuhan 430015, China)

机构地区：[1]武汉市疾病预防控制中心,武汉430015 [2]武汉市妇女儿童医疗保健中心,武汉430015

出　　处：《中国食物与营养》2016年第12期22-26,共5页Food and Nutrition in China

基　　金：武汉市卫生局资助课题(项目编号:WG11B02)

摘　　要：目的：分析高效液相色谱法（HPLC）同步测定多种色素含量的不确定度。方法：通过评定各不确定度分量,计算合成标准不确定度及扩展不确定度。结果：HPLC同步测定多种色素含量的扩展不确定度范围在3.97%~7.13%（k=2）;天平称量和提取回收率对合成色素的测量结果不确定度影响最大。结论：本研究建立了简便易行的HPLC同步测定肉制品中色素含量检测结果的不确定度评定方法。Objective To evaluate the uncertainty of the content of several kinds of synthetic pigments for simultaneous determination by high performance liquid chromatography（ HPLC）. Method The synthetic standard uncertainties and expanded uncertainties were calculated. Result It was found that sample weighing and the rate of recovery had the strongest impact on the uncertainty. The uncertainties of synthetic pigments were at the range of 3. 97% ~ 7. 13 %（ k = 2）. Conclusion A feasible method for uncertainty evaluation on the determination on synthetic pigments in processed meat was established.

关 键 词：高效液相色谱 同步测定 合成色素 不确定度 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]