青天葵4种提取物抗氧化活性与其总黄酮和总酚含量的相关性  

Correlations between antioxidant activities and contents of total flavonoids and total phenols of four extracts from Nervilia fordii

作　　者：许海棠[1,2] 马少妹[2] 张金彦[2] 

机构地区：[1]广西民族大学,广西高校微生物与植物资源利用重点实验室,广西南宁530008 [2]广西民族大学化学化工学院,广西南宁530008

出　　处：《中成药》2017年第1期121-125,共5页Chinese Traditional Patent Medicine

基　　金：国家民委科研项目(14GXZ012);广西民族大学校级项目(2012MDZD042,2014MDQD015)

摘　　要：目的考察青天葵Nervilia fordii(Hance)Schltr.氯仿、乙酸乙酯、正丁醇、乙醇提取物抗氧化活性与其总黄酮和总酚含有量的相关性。方法 Al Cl3-HAc-Na Ac显色法和福林-酚比色法分别测定青天葵4种提取物中总黄酮和总酚含有量,通过测定DPPH自由基清除能力、羟自由基清除能力、ABTS自由基清除能力、还原能力评价提取物抗氧化活性,SPSS17.0软件分析抗氧化活性与2种成分含有量的相关性。结果青天葵4种提取物的抗氧化活性均呈量效关系,乙酸乙酯提取物最强,而乙醇提取物最弱。其与总黄酮含有量有一定相关性(0.604<r<0.638),与总酚含有量相关性显著(r>0.977)。结论青天葵乙酸乙酯提取物可用于天然抗氧化剂开发,总酚含有量是影响其抗氧化活性的主要因素。AIM To investigate the correlations between antioxidant activities and contents of total flavonoids and total phenols of chloroform,ethyl acetate,n-butanol and ethanol extracts from Nervilia fordii（ Hance） Schltr.. METHODS The contents of total flavonoids and total phenols in four extracts from N. fordii were determined by Al Cl3-HAc-Na Ac coloration method and Folin-Ciocalteu colorimetric method,respectively. The antioxidant activities of extracts were evaluated by determining scavenging activities of DPPH radical,hydroxyl radical and ABTS radical,together with reducing power. Then the correlations between antioxidant activities and contents of two constituents were analyzed by SPSS17. 0 software. RESULTS The antioxidant activities of four extracts from N. fordii all showed dose-effect relationships,that of ethyl acetate extract was the strongest,while that of ethanol extract was the weakest. They had some correlations with the content of total flavonoids（ 0. 604 r 0. 638） and significant correlations with the content of total phenols（ r〉0. 977）. CONCLUSION Ethyl acetate extract from N. fordii can be used for the development of natural antioxidants,and the content of total phenols is the main factor to influence its antioxidant activity.

关 键 词：青天葵 提取物 总黄酮 总酚 抗氧化活性 相关性 

分 类 号：R284.1[医药卫生—中药学]