基于SSR标记的黔南茶树种质资源DNA指纹图谱构建  

Establishment of DNA Fingerprinting for Tea Germplasm from Qiannan Prefecture by SSR Markers

作　　者：陈世军[1] 张明泽[1] 姚玉仙[1] 谢维斌 CHEN Shi-jun ZHANG Ming-ze YAO Yu-xian XIE Wei-bin(Qiannan Normal University for Nationalities ,Duyun 558000 Duyun Forestry Bureau of Guizhou Province ,Duyun 558000)

机构地区：[1]黔南民族师范学院生物科学与农学院,都匀558000 [2]贵州省都匀市林业局,都匀558000

出　　处：《植物遗传资源学报》2017年第1期106-111,共6页Journal of Plant Genetic Resources

基　　金：贵州省普通高校教育质量提升重点科研项目(2011017);黔南民族师范学院项目(QNSY2010014)

摘　　要：以黔南60个野生茶树种质资源为材料,使用15对引物,通过SSR技术进行了DNA指纹数据库的构建。结果显示,所采用的15对引物共扩增出147个等位基因,有较好的多态性。位点的期望杂合度和多态性信息含量的变化范围分别为0.128~0.939和0.124~0.927,平均值分别为0.602和0.572。综合各项指标筛选出6对引物QNSSR01、QNSSR02、QNSSR04、QNSSR06、QNSSR18、QNSSR23上的23个等位基因用于黔南茶树种质资源DNA指纹图谱构建,60个茶树种质资源的SSR指纹图谱互不相同,可以作为各材料特定的图谱。研究结果为黔南茶树种质资源保护和品种创新利用奠定了基础。Sixty tea germplasm from Qiannan prefecture were collected to construct DNA fingerprint database by SSR technique. The results showed that 147 alleles were detected with considerable polymorphism by 15 pairs of primers employed in the experimental design. The ranges of expected heterozygosities and polymorphism information content at 15 SSR loci of 60 cultivars were 0. 128-0. 939 and 0. 124-0. 927 respectively ,with an average of 0. 602 and 0. 572 respectively. And a total of selected 23 alleles from 6 primers （ QNSSR01, QNSSR02, QNSSR04, QNSSR06 ,QNSSR18 ,and QNSSR23 ）were used for the DNA fingerprint construction. Sixty tea accessions with different SSR fingerprints of each other can serve as the cultivars-specific patterns and as an important basis for cuhivars identification.

关 键 词：黔南 茶树资源 SSR DNA指纹图谱 

分 类 号：S571.1[农业科学—茶叶生产加工]