婴儿喂养方式的改变对肠道菌群多样性的影响  

Effects of feeding pattern changes on intestinal microflora diversity

作　　者：贾宏信[1] 苏米亚[1] 陈文亮[1] 刘翠平[1] 齐晓彦[1] 

机构地区：[1]光明乳业股份有限公司乳业生物技术国家重点实验室上海乳业生物工程技术研究中心,上海200436

出　　处：《中国微生态学杂志》2016年第12期1365-1369,共5页Chinese Journal of Microecology

基　　金：上海市经委引进技术的吸收与创新计划(14XI-1-15);科技部农业科技成果转化资金(2012GB2CO00141)

摘　　要：目的分析婴儿喂养方式的改变对肠道菌群的影响。方法招募45名（月龄1-3个月）志愿者,随机分为三组（母乳组：母乳喂养;对照组：干预后市售普通配方奶粉喂养;产品组：干预后试验配方奶粉喂养）,利用平板计数和PCR-DGGE（变性梯度凝胶电泳）技术分析婴儿干预前和干预后其肠道菌群的变化。结果饮食干预后,婴儿肠道内双歧杆菌的数量,母乳组和产品组比较差异无统计学意义（t=0.510,P=0.614）,产品组和对照组比较差异存在统计学意义（t=2.541,P=0.016）;婴儿肠道内微生物的多样性指数,母乳组和产品组都显著增高（P≤0.05）。结论产品组配方奶粉是一款利于婴幼儿肠道有益菌增殖的产品。Objective To study the effect of feeding pattern on infants＇ intestinal flora. Methods 45 volunteers （aged 1 to 3 months） were enrolled and randomly divided into three groups to accept breastfeeding （Breastfeeding group）, commercial infant formula （Control group） or the tested infant formula （Product group） respectively. Plate counting and PCR-DGGE （polymerase chain reaction-denaturing gradient gel electrophoresis） technology were used to analyze the intestinal flora of the infants before and after the diet intervention. Results No statistical difference （t=O. 510, P=0. 614） was found between the product group and breastfeeding group in bifidobacteria count after diet intervention, but between the product group and control group was statistical difference （t： 2. 541, P=0. 016） . The indexes of intestinal flora diversity in the product group and breastfeeding group significantly increased （P=0.05） . Conclusion The tested infant formula has positive effect on the growth of intestinal probiotic bacteria.

关 键 词：肠道菌群 婴儿 PCR-DGGE 婴幼儿配方食品 

分 类 号：R378[医药卫生—病原生物学]