二仙汤对卵巢切除大鼠棕色脂肪产热相关蛋白表达的影响  

Effects of Erxian Decoction on Expression Levels of Thermogenesis-related Proteins in Brown Adipose Tissue of Ovariectomized Rats

作　　者：曾宁溪 关莉[1] 周乐全[1] 刘海梅[1] 李小英[1] 薛倩倩[1] 徐进文[1] 李润美[1,2] ZENG Ningxi GUAN Li ZHOU Lequan LIU Haimei LI Xiaoying XUE Qianqian XU Jinwen LI Runmei(School of Basic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, China School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, China)

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]广州中医药大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2017年第1期1-5,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金青年基金项目(814014319;81202718);广东省高校优秀青年教师培养计划(yq2014045)

摘　　要：目的研究二仙汤对卵巢切除大鼠肩胛区棕色脂肪中产热相关蛋白表达的影响。方法 40只雌性SD大鼠随机分为5组,分别为假手术组(SHAM),卵巢切除模型组(OVX),二仙汤低剂量治疗组(EXDL),二仙汤高剂量治疗组(EXDH),雌激素治疗组(E),并分别给予相应的药液连续灌胃给药14周。检测各组大鼠血脂4项;HE染色观察各组大鼠肩胛区棕色脂肪形态学变化;应用Western-blot及q PCR检测各组大鼠肩胛区棕色脂肪中脱偶联蛋白-1(UCP-1)、过氧化物酶体增殖物激活受体γ辅激活因子-1α(PGC-1α)的蛋白及其m RNA的表达水平。结果卵巢切除模型大鼠体质量、腹腔内脂肪质量与假手术组大鼠相比显著增加,卵巢切除大鼠血清总胆固醇水平较假手术组大鼠显著升高;二仙汤治疗后可降低卵巢切除大鼠体质量及腹腔内脂肪质量并降低血清总胆固醇水平。卵巢切除大鼠肩胛区棕色脂肪中UCP-1及PGC-1α的蛋白及其m RNA的表达水平下降,二仙汤治疗后可提高卵巢切除大鼠肩胛区棕色脂肪中UCP-1及PGC-1α的蛋白及其m RNA的表达水平。结论二仙汤可降低卵巢切除大鼠的体质量和腹腔内脂肪的质量并降低血清中总胆固醇的水平,这可能与其提高卵巢切除大鼠肩胛区棕色脂肪中产热相关蛋白UCP-1及PGC-1α的蛋白及其m RNA表达水平有关。Objective To investigate the effects of Erxian Decoction（ED）on the expression levels of thermogenesisrelated proteins in interscapular brown adipose tissue of ovariectomized rats. Methods Forty female SD rats were randomly divided into sham operation group, ovariectomized group, estrogen group, and low- and high-dose ED groups,which were treated with normal saline,estrogen,ED respectively for 14 weeks. Serum lipids levels were tested. By using HE staining,the morphological features of interscapular brown adipose tissue of rats in each group were observed. The protein and m RNA expression levels of uncoupling protein-1（UCP-1）and peroxisome proliferatoractivated receptor co-activator 1α（PGC-1α）in rat interscapular brown adipose tissue of each group were determined by Western blot and q PCR. Results Compared with sham operation group,rats in ovariectomized group had high body weight, intra-abdominal fat weight and serum cholesterol level. ED treatment was able to decrease body weight,intra-abdominal fat weight and serum cholesterol level in ovariectomized rats. The protein and m RNA levels of UCP-1and PGC-1α in interscapular brown adipose tissue of ovariectomized rats were significantly reduced, and ED treatment could increase the protein and m RNA levels of UCP-1 and PGC-1α in ovariectomized rats. Conclusion ED treatment is effective on decreasing the body weight, interscapular fat weight and serum total cholesterol level in ovariectomized rats. The mechanism is probably associated with the up-regulation of protein and m RNA expression levels of thermogenesis-related proteins UCP-1 and PGC-1α in interscapular brown adipose tissue of ovariectomized rats.

关 键 词：二仙汤 棕色脂肪 绝经 脱偶联蛋白-1 

分 类 号：R285.5[医药卫生—中药学]