多不饱和脂肪酸结构酯酶法合成工艺的研究  

Study on enzymatic synthesis process of polyunsaturated fatty acid structured esters

作　　者：刘爽 杨洋[2] 聂开立 王萌[2] 邓利[2] 

机构地区：[1]开封高级中学,开封475003 [2]北京化工大学生命科学与技术学院,北京100029 [3]厦门北化生物产业研究院有限公司,厦门361026

出　　处：《生物产业技术》2017年第1期105-110,共6页Biotechnology & Business

基　　金：国家自然科学基金(21476017);北京化工大学学科建设项目基金(XK1537)

摘　　要：运用低温溶剂结晶法富集藻油中多不饱和脂肪酸,最佳结晶条件为-20℃静置结晶72h,丙酮溶剂与藻油的体积质量比30:1(v/wt,m L/g)。在此条件下,藻油sn-2位的脂肪酸组成中PUFA含量从74.8%提高到92.2%。以富集后的藻油和癸酸为底物,合成富含PUFA的结构酯,优化了酸解反应条件:以脂肪酶Ls-20为催化剂,以正己烷为反应介质,底物藻油与癸酸的摩尔比1:10,酶用量10%(占底物藻油的质量百分比),氮气保护,40℃,180rpm反应25h。利用低温溶剂结晶法分离纯化酸解产物,在丙酮与油脂的体积质量比1.6;1(v/wt,m L/g),-20℃下结晶24h,结构酯回收率达到78.7%。Using low temperature solvent crystallization to enrich polyunsaturated fatty acids from algae oil and the optimal crystallization conditions were acetone vs algae oil ratio 30：1（v/wt, mL/g）, -20℃ and 72h. The content of PUFA in the sn-2 position fatty acid of algae oil was increased from 74.8% to 92.2% under the optimal conditions. Structured lipid with enriched PUFA was synthesized by utilizing the extracted algae oil and capric acid as substrates. The optimized conditions for acidolysis reaction were lipase Ls-20 as biocatalyst, hexane as reaction medium, algae oil vs capric acid ratio 1：10, nitrogen protection, 40℃ , 180rpm and 25h. Employing low temperature solvent crystallization to purify the products, recovery rate of structured esters reached 78.7% under the conditions of acetone vs modified algae oil ratio 1.6∶1（v/wt, mL/g）, -20℃ and 24h.

关 键 词：多不饱和脂肪酸结构酯 脂肪酶 酸解反应 低温溶剂结晶 结构酯 

分 类 号：TQ645.6[化学工程—精细化工]