实时直接分析-串联质谱法测定葡萄酒中赭曲霉毒素A  

Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry

作　　者：宫小明[1] 马荣桧 王洪涛[1] 郭礼强[1] 李凯[1] 吴振兴[2] 赵晗[1] 孙军[1] 

机构地区：[1]潍坊出入境检验检疫局,山东潍坊261041 [2]山东出入境检验检疫局检验检疫技术中心,山东青岛266002

出　　处：《色谱》2017年第2期185-190,共6页Chinese Journal of Chromatography

基　　金：国家质检总局科研基金资助项目(2013IK177)~~

摘　　要：建立了葡萄酒中赭曲霉毒素A的实时直接分析-串联质谱(DART-MS/MS)方法。前处理采用乙酸乙酯提取,二甲基十八碳硅烷粉(ODS)粉分散固相萃取净化,采用DART-MS/MS检测,同位素稀释内标法定量。结果表明:在1.0、2.0、10μg/kg 3个加标水平下,回收率为88.7%~105.7%,RSD为8.5%~12.8%,定量限为0.5μg/kg。该方法具有简单、快速、灵敏度高等特点,能满足葡萄酒中赭曲霉毒素A检测的要求。A method for the determination of ochratoxin A (OTA) in wine was developed using direct analysis in real time. electrospray ionization tandem mass spectrometry (DART-MS/MS). The sample was extracted with ethyl acetate and purified by votexing with ODS powder, detected using DART-MS/MS. The results showed that the average recoveries ranged from 88.7% to 105.7% with the RSDs of 8.5% -12.8% at three spiked levels of 1.0, 2.0, and 10 mu g/kg. The LOQ was 0.5 mu g/kg. The proposed method was accurate, rapid and reliable, which can be successfully applied to the qualitative and quantitative analysis of ochratoxin A in wine.

关 键 词：QUECHERS 同位素稀释内标 实时直接进样分析 串联质谱法 赭曲霉毒素A 葡萄酒 

分 类 号：O658[理学—分析化学]