研磨法破碎小球藻细胞工艺优化  

Optimization of Chlorella cell disruption process by grinding method

作　　者：鲁龙[1] 毕生雷 金洪波[1] 吴娟[1] 张鹏飞[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000

出　　处：《中国酿造》2017年第1期116-119,共4页China Brewing

基　　金："十二五"农村领域国家科技计划(2011BAD14B05)

摘　　要：选取破碎过程中的关键因素,以油脂得率为评价指标,通过进行单因素试验和正交试验,对研磨法破碎小球藻细胞的工艺条件进行了研究。结果表明,小球藻细胞破碎最佳工艺条件为藻液质量浓度150 g/L,破碎时间2.5 h,球磨机转速400 r/min,藻液与钢珠的质量比为3:4。在此工艺条件下,能达到较好的破碎效果,油脂得率为46.89%。The key factors were selected in the process of disruption. Using oil yield as evaluation index, the process conditions of Chlorella cell dis- ruption by grinding method were researched through the single factor and orthogonal experiments. The results indicated that the optimum disruption conditions were optimized as follows： Chlorella liquid concentration 150 g/L, disruption time 2.5 h, rotate speed of ball grinder 400 r/rain, the mass ratio of Chlorella liquid and steel ball 3：4. Under the conditions, the disruption effect was better, and the oil yield was 46.89%.

关 键 词：小球藻 研磨法 破碎 油脂得率 

分 类 号：TK6[动力工程及工程热物理—生物能]