玉米须提取液抗氧化功能特性的研究  

Study on antioxidative activity of the extract of corn silk

作　　者：郭皓[1] 关宏[1] 侯惠玲[1] 刘振艳[1] 陈雪[1] 刘韩[1] 藏传刚[1] 刘宇超[1] 杨文钦[1] 

机构地区：[1]齐齐哈尔医学院医药科学研究院,黑龙江齐齐哈尔161006

出　　处：《中国临床药理学杂志》2017年第3期258-261,共4页The Chinese Journal of Clinical Pharmacology

基　　金：齐齐哈尔市科学技术局计划基金资助项目(SFGG-201335);齐齐哈尔医学院院内科研基金资助项目(QY2015Q-04)

摘　　要：目的通过体外抗氧化实验评估玉米须提取液的体外抗氧化活性。方法用硫酸-酚法、二硝基水杨酸法(DNS法)及凯氏定氮法检测出玉米须提取液总糖、还原糖及蛋白质含量;用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测玉米须提取液中蛋白质的组成成分;用ABTS·+阳离子自由基清除率及氧自由基吸收能力实验评估玉米须提取液体外抗氧化活性。结果玉米须提取液中总糖、还原糖及蛋白质的含量分别为51.70%,10.43%及21.80%。玉米须提取液中含有可溶性蛋白,其分子量在9.5~20.0 k D。当玉米须提取液浓度为0.40 mg·m L^(-1)时,ABTS·^+阳离子自由基清除率已达70.29%,玉米须提取物清除ABTS阳离子自由基的半抑制率(IC_(50))为0.23 mg·m L^(-1)。玉米须提取液的相对氧化自由基吸收能力值(ORAC)为3.40mmol TE·g^(-1),相当于维生素C自由基吸收能力的1/3。结论玉米须提取液具有较强的体外抗氧化活性。Objective To investigate the main components and antioxidant activity of the extract of corn silk. Methods The extraction of proteins was determined by the Kjeldahl method,total sugar was determined by the phenol- sulfuric acid assay,reducing sugar by 3,5- dinitrosalicylic acid（ DNS） assay,respectively. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis assay（ SDS- PAGE） profile was used to show the composition of the protein of the extract of corn silk. The antioxidant activity of the extract of corn silk was evaluated by the ABTS·＋radical scavenging rate and oxygen free radical absorption capacity.Results The contents of total sugar,reducing sugar and protein were51. 70%,10. 43% and 21. 80%,respectively. SDS- PAGE profile showed that the extract of corn silk contained soluble protein,and its molecular weight was between 9. 5 k D and 20 k D. The experimental results showed that the free radical scavenging rate of ABTS ·＋had reached 70. 29% when the extract concentration was 0. 40 mg · m L^（- 1）.In the reaction system,the half inhibition rate（ IC_（50）） of ABTS free radical scavenging activity was 0. 23 mg·m L（- 1）. The total antioxidant activities evaluated by ORAC assay were 3. 40 mmol Trolox equivalents·g（- 1）,equivalent to 1 /3 of absorption capacity of Vitamin C free radical.Conclusion The extract of corn silk has prominent antioxidant activity.

关 键 词：玉米须 抗氧化活性 自由基 半抑制率 

分 类 号：R28[医药卫生—中药学]