纳米铁对蚯蚓蛋白质含量和抗氧化酶系的影响  

Effect of Nano-scale Zero-valent Iron on Protein Content and Antioxidase Enzyme Activities in Eisenia Foetida

作　　者：陈旭[1] 李春江 范志云[1] 庞伟玲 李铁龙[3] 

机构地区：[1]天津市水质科学与技术重点实验室/天津城建大学环境与市政工程学院,天津300384 [2]中国石油华北石化公司,河北任丘062552 [3]南开大学环境科学与工程学院/环境污染过程与基准教育部重点实验室/天津市生物质类固废资源化技术中心,天津300071

出　　处：《环境科学与技术》2016年第11期26-30,共5页Environmental Science & Technology

基　　金：国家自然科学基金(41173102)

摘　　要：采用人工土壤法,研究了不同暴露浓度下纳米铁对赤子爱胜蚓（Eisenia foetida）体内蛋白质含量和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）等抗氧化酶活性的影响。结果表明：当纳米铁浓度在10~500 mg/kg时,纳米铁对蚯蚓体内蛋白质含量未产生显著性影响（P〉0.05）。2 000 mg/kg组比对照组蛋白质含量有明显增加,最高时比对照组增加了74%。蚯蚓体内CAT、SOD和GSH-PX对纳米铁浓度响应不同,在10~100 mg/kg时CAT、SOD和GSH-PX均无响应,当纳米铁浓度在500~2 000 mg/kg时这3种酶的活性变化趋势基本一致,都呈现先增加后降低的趋势。Impact of different exposure concentrations of nano-scale zero-valent iron（n ZVI）to the protein content and the enzyme activity of superoxide dismutase（SOD）,catalase（CAT） and glutathione peroxidase（GSH-PX） in Eisenia foetida by the method of artificial soil was studied. Results showed that when n ZVI concentrations were 10 ~500 mg/kg,protein content of earthworm remained the same as the control group（P〉0.05）.When n ZVI concentration was up to 2 000 mg/kg,protein content increased significantly compared with that of the control,the highest increased by 74% compared with the control group. n ZVI concentrations had different effects on the CAT,SOD and GSH-PX activities,and n ZVI had no obviously effect on activities of three antioxidase enzymes at 10 ~100 mg/kg. The activity trend of SOD was generally in accord with that of the activity of CAT and GSH-PX at 500 ~2 000 mg/kg. The activities of three antioxidase enzymes increased first and then decreased,while it had no obvious regularity in the increase of n ZVI＇s concentration.

关 键 词：纳米铁 赤子爱胜蚓 蛋白质含量 抗氧化酶 

分 类 号：X171.5[环境科学与工程—环境科学]