适配体上转换发光生物传感技术在食品安全快速检测中的应用  

Application of aptamer-based upconverting phosphor biosensing technology in rapid detection for food safety

作　　者：任舒悦 李巧凤[2] 高志贤[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050 [2]郑州大学公共卫生学院,450001

出　　处：《国际生物医学工程杂志》2016年第6期362-367,共6页International Journal of Biomedical Engineering

基　　金：国家自然科学基金（21477162）;天津市科技支撑计划项目（14ZCZDSF0021）

摘　　要：食品安全快速检测技术在预防医学领域扮演着至关重要的角色。传统理化检测方法存在耗费大、操作难、检测环境要求高等问题，不能满足质量安全监督中快速现场筛查的需求，正逐步被新兴的快速检测传感技术代替。新型适配体上转换发光生物传感技术建立在功能化上转换发光纳米材料与适配体技术相结合基础上，是一种新兴快速检测技术。与传统免疫荧光技术相比，其灵敏度高、特异性好、稳定性强，广泛应用于快速检测中。对上转换发光技术及适配体技术进行了简要介绍，对基于适配体的上转换发光生物传感技术及其在食品安全检测领域的应用进行了综述；根据目前的应用领域，指出了该技术存在的瓶颈及未来的发展趋势，为该技术在食品安全及多领域的应用提供了参考。The rapid detection technology in food safety plays a vital role in the field of preventive medicine. The traditional physical and chemical detection methods have some limitations, such as costly, difficult to operate and high requirements on environment, can not meet the needs of quality and safety on-site rapid screening, and are gradually replaced by emerging sensor detection technologies. The aptamer-upconversion biosensor technology is a novel rapid detection technology, which is based on the combination of functionalized upconversion nanomaterials and aptamer technology. Compared with the traditional immunofluorescence technology, it has advantages in sensitivity, specificity and stability, and is widely used in rapid detection. In this paper, the upconversion luminescence and aptamer technologies were briefly introduced, and the aptamer-based upconverting phosphor biosensing technology and its application in rapid detection of food safety were summarized. Based on the current research status, the bottleneck and the future development trend of this technology were analyzed, which provides a reference for the application of this technology in food safety and other fields.

关 键 词：适配体 上转换发光 生物传感器 食品安全 快速检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]