产丁醇梭菌基因改造的研究进展  

Research Progress for Genetic Modification of Butanol-producing Clostridia

作　　者：张超[1] 王义强[1] 王启业[1] 黄瑞春 米小琴 ZHANG Chao WANG Yi-qiang WANG Qi-ye HUANG Rui-chun MI Xiao-qin(Key Lab of Non-wood Forest Nurturing and Protection of National Ministry of Education/Central South University of Forestry and Technology, Biotechnology Laboratory/Central South University of Forestry and Technology, Changsha 410004 Xiangxi Forest Ecological Research Station, Xiangxi 416000)

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室中南林业科技大学生物技术实验室,长沙410004 [2]湘西州森林生态研究实验站,湘西416000

出　　处：《生物技术通报》2017年第1期106-113,共8页Biotechnology Bulletin

基　　金：国家林业局"948"项目(2011-4-13)

摘　　要：产丁醇梭菌作为丁醇发酵的主要生产菌,近年来成为研究的热点,而丁醇作为新型可再生能源,其优势远大于乙醇,因此,对产丁醇梭菌的基因改造研究具有重大意义。从关键基因、糖酵解途径和丁醇耐受性的角度出发,介绍了近几年来对产丁醇梭菌基因改造的研究进展。讨论了目前研究存在的问题,并对如何提高丁醇产量提出一些见解,旨为广大研究者提供新的思路。Butanol-producing Clostridia as butanol fermentation strains have been studied frequently in recent years. As a new renewableenergy sources,butanol has obviously more advantages than ethanol. Therefore,it is of great significance to study gene modification of butanolproducing Clostridia. In this review,from three aspects of key genes,glycolytic pathway,and butanol tolerance,we introduced the latestresearch progress on gene modification of butanol-producing Clostridia. Meanwhile,we discussed the issues in the current research,and putforward suggestions on how to improve the butanol yield,aiming at providing some new ideas for the researchers.

关 键 词：产丁醇梭菌 ABE发酵 EMP 基因过表达 基因失活 丁醇耐受性 

分 类 号：TQ923[轻工技术与工程—发酵工程]