不同提取方法对大花红景天多糖的DPPH自由基清除能力的影响  

The Effect of DPPH Free Radical Scavenging Activity on the Polysaccharides from Rhodiola Crenulata with the Different Extraction Ways

作　　者：周凌云[1] 胡颖[2] 唐博安 ZHOU Ling-yun HU Ying TANG Bo-an(School of Pharmacy, Wannan Medical College, Wuhu,Anhui 241002, China)

机构地区：[1]皖南医学院药学院 [2]皖南医学院,安徽芜湖241002

出　　处：《通化师范学院学报》2017年第2期18-21,共4页Journal of Tonghua Normal University

基　　金：安徽省大学生创新创业训练计划(AH201410368124);皖南医学院大学生科研资助(WK2014S23)

摘　　要：目的:研究不同提取方法对大花红景天多糖的DPPH自由基清除能力的影响.方法:以文献及前期实验优化条件,采用热水浸提法、微波提取法、超声辅助水提法和超声辅助碱提法对大花红景天进行提取,提取物以1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法评价多糖的自由基清除能力.结果:不同提取方法对DPPH自由基清除能力为超声辅助碱提法>超声辅助水提法>热水浸提法>微波提取法.结论:利用超声辅助碱提大花红景天多糖,其多糖得率及对DPPH自由基清除能力均较高.Objects ： To study the effect of DPPH free radical scavenging activity on the polysaccharides. Meth- ods：the polysaccharides from R. Crenulata were extracted by the hot water, the microwave, the ultrasonic wave-assisted with water, and the ultrasonic wave-assisted with alkali, and the free radical scavenging activity of all polysaccharides were evaluated by 1,1-diphenyl-2-picrylhydrazyl （DPPH）. Results：the scavenging activity of DPPH free radical： polysaccharides with the ultrasonic wave-assisted alkali extraction 〉 polysaccharides with the ultrasonic wave-assisted water extraction 〉 polysaccharides with hot water extraction 〉 polysaccharides with the microwave extraction. Conclusion： the extraction yield and the scavenging activity of DPPH free radical of R. crenulata polysaccharide with the ultrasonic wave-assisted alkali extraction were higher than those with other extraction ways.

关 键 词：大花红景天 多糖 不同提取方法 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]