蛹虫草液体发酵过程主要活性成分的代谢变化  

Metabolism Changes of Main Active Ingredients During Liquid Fermentation of Cordyceps militaris

作　　者：曹灵杰 卢雪梅[1] 金小宝[1] 朱家勇[1] 

机构地区：[1]广东药科大学基础学院药用生物活性物质研究所/广东省生物活性药物研究重点实验室,广东广州510006

出　　处：《中药材》2016年第9期1966-1970,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:研究蛹虫草在液体发酵过程中主要活性成分的累积与代谢变化。方法:选取蛹虫草菌种GIM5.270,在基本发酵条件下延长发酵时间至20 d,通过含量分析方法检测每24 h时间间隔后生物量、虫草多糖、虫草酸、腺苷、虫草素在发酵体系中的累积与动态变化。结果:复合氮源组成的基础培养基比单一氮源组成所产生的生物量更高,随着时间发展,菌丝体生物量越来越高,虫草多糖与虫草酸(D-甘露醇)先增高后降低,腺苷逐渐降低,虫草素(3-脱氧腺苷)逐渐增高。结论:随着时间的发展,整个体系会吸收自身产生的营养物质出现自溶现象,本研究探索了主要活性成分在发酵体系中的代谢变化,确定了主要活性成分的最佳收集时间,为提高活性成分的产率提供依据。Objective： To study the accumulation and changes of main active ingredients during liquid fermentation of Cordyceps militaris. Methods： The militaris varity GIM5. 270 was selected to extended fermentation time to 20 days on the basic fermentation condition. Meanwhile,the accumulation and dynamic changes of biomass,polysaccharide,cordycepic acid,adenosine and cordycepin in the fermentation system were detected by the analytical method of contents per 24 hour. Results： The foundation culture medium composed of complex nitrogen sources could reach a higher biomass level than single nitrogen sources. In addition,with the development of time,the mycelial biomass increased,the contents of polysaccharide and cordycepic acid（ D-mannitol） increased firstly and then decreased,the contents of adenosine decreased gradually and cordycepin（ 3-deoxy adenosine） increased gradually. Conclusion： The whole system is observed autolyzed phenomenon caused by absorbing self-generated nutrients. In this study,the dynamic changes of the main active ingredients in the fermentation system are researched and the optimum collecting time is determined,which provides evidence for reaching a better yield of the active ingredients.

关 键 词：蛹虫草 发酵 活性成分 含量 

分 类 号：R282.71[医药卫生—中药学]