氟甲喹胶体金免疫层析试纸条研究  

Study on the Application of Colloidal Gold Labled Immunochromatographic Test Strip for the Determination of Flumequine

作　　者：刘冰[1] 童贝[1] 生威[1] 张燕[1] 张富源[1] 冯久慧 王硕[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品研究与开发》2016年第22期115-118,共4页Food Research and Development

基　　金：国家“十二五”科技支撑计划项目(2013BAD18B11);国家自然科学基金青年科学基金(31301462);国家国际科技合作专项项目(2014DFR30350)

摘　　要：选择氟甲喹(FLU)作为目标物,制备能够特异性识别氟甲喹的多克隆抗体,建立氟甲喹的胶体金标记免疫层析分析方法。胶体金颗粒的粒径为20 nm;金标抗体连接的最佳p H值为8.5、最适抗体量为20μg/m L;捕获抗体的包被浓度为0.5 mg/m L,二抗1∶200倍稀释,选择Milllipore HF135s硝酸纤维膜作为固相载体,金标抗体1∶10稀释喷涂到金标垫上;在最优条件下组装试纸条,将金标抗体铺在结合释放垫上的组装方式其方法检出限为20μg/L;选择鸡肉、牛肉、猪肉、鱼肉、虾肉、猪肝和牛奶7种实际样品进行添加回收试验,样品检出限为50μg/L。In this study,flumequine（FLU）was chosen as the target,colloidal gold labled immunochromatographic strip was developed based on the specifical polyclonal antibody of flumequine. Colloidal gold particles size was 20 nm;the optimum p H of gold-labeled antibody was 8.5,the optimal amount of antibody was 20 μg/m L;capture antibody concentration was 0.5 mg/m L,sheep anti-rabbit secondary antibody was diluted to 1 ∶ 200,choosed Millipore HF135 s models of nitrocellulose membrane as solid phase carrier,gold-labeled antibody was diluted 1 ∶ 10 sprayed gold pad;under optimal conditions assembled test strip,the method detection limit was20 μg/L by spaying gold labled antibody in its release pad;the seven kinds of real samples chicken,beef,pork,fish,shrimp,liver and milk spiked experiment,the sample detection limit was 50 μg/L.

关 键 词：氟甲喹 胶体金 免疫层析试纸条 

分 类 号：R155.5[医药卫生—营养与食品卫生学]