细胞膜ATPase活性和膜脂肪酸组成对植物乳杆菌耐酸性的影响  

Effect of Membrane's ATPase Activity and Lipid Profile on the Acid Tolerance of Lactobacillus plantarum

作　　者：田丰伟[1] 尹义敏 翟齐啸 王刚[1] 张秋香[1] 张灏[1] 陈卫[1,2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国食品学报》2016年第12期17-22,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31371721);国家科技支撑计划(2013BAD18B01);江苏自然科学基金项目(BK20131102)

摘　　要：以植物乳杆菌CCFM8661及其酸耐受突变株LPV-30、LPV-48为研究对象,通过考察酸胁迫对其生理应激反应的影响而探索其可能的耐酸机制。研究结果表明:酸胁迫引起H^+-ATPase活性提高,胞内ATP含量降低,植物乳杆菌利用H^+-ATPase,通过消耗胞内ATP,将胞内H^+排出,从而保持胞内pH的动态平衡。与原始菌株相比,突变菌株都保持了较高的H^+-ATPase活性和胞内ATP水平。细胞膜脂肪酸分析表明:酸胁迫引起总饱和脂肪酸含量的减少,不饱和度和单不饱和脂肪酸的含量增加,并且突变菌株保持较高的饱和脂肪酸含量。本研究结果有助于了解植物乳杆菌的酸胁迫抗性机制。In this paper, the effects of acid stress on physiological responses were investigated by using Lactobacillus plantarum CCFM8661 and its mutant strains LPV-30 and LPV-48 as initial strain to preliminary study its acid resistant mechanism. The results showed that acid stress led to the increase of H^＋-ATPase activity and the decrease of ATP content, and the mutants kept higher H^＋-ATPase and intracellular ATP pool than the parental strains. The mutants could utilize the H^＋-ATPase to discharge H^＋through the consumption of intracellular ATP to maintain pH homeostasis. Results of the cell membrane fatty acid contents showed that acid stress caused the decrease of total saturated fatty acids and the increase of the U/S ratio and monounsaturated fatty acids content, moreover, mutant stains LPV-30 and LPV-48 possessed higher saturated fatty acids content. Results presented in this study provided theoretical basis for further elucidation of the acid tolerance mechanisms employed by Lactobacillus plantarum.

关 键 词：植物乳杆菌 酸胁迫 H^+-ATPASE活性 ATP含量 膜脂肪酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]