谷氨酸水解原人参二醇组皂苷制备人参皂苷Rg_3工艺的优选  

Process optimization of ginsenoside Rg_3 prepared by hydrolysing protopanaxadiol-type saponins by glutamic acid

作　　者：夏娟[1,2] 张金秋[1,2] 阮长春[1] 李伟[2] 刘志[1] 

机构地区：[1]吉林农业大学农业现代化综合技术研究所,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《上海中医药杂志》2017年第1期102-106,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(31201331);吉林省高校优秀青年科研人才春苗培育计划资助项目(201448)

摘　　要：目的研究谷氨酸水解原人参二醇组皂苷制备人参皂苷Rg_3的最佳工艺。方法以谷氨酸为催化剂,原人参二醇(PPD)组皂苷为原料,通过单因素考察和正交试验对人参皂苷Rg_3的制备工艺进行优化,反应产物用HPLC进行定量分析。结果正交试验优化结果表明,原人参二醇组皂苷(10 g/L)和谷氨酸(10 g/L)于80℃反应2 h,原人参二醇组皂苷被全部降解,人参皂苷Rg_3的转化率可达65.12%。结论该法操作简单,成本低,对环境无污染,是一种快速制备20(S)-Rg_3和20(R)-Rg_3的可行方法。Objective To study the optiraum process of ginsenoside Rg3 prepared by hydrolysis protopanaxadiol-type saponins by glutamic acid. Methods Taken glutamic acid as catalyst and protopanaxadiol（ PPD ）-type saponins as raw material, the preparation condition of ginsenoside Rg3 was optimized by single factor investigation and orthogonal test,and the reaction products were quantitatively analyzed by HPLC. Results The optimization results of orthogonal test showed that all PPD-type saponins were converted when PPD-type saponins （ 10 g/L） were hydrolyzed by glutamic acid （ 10 g/L） at 80℃ for 2 h,the conversion rate of ginsenoside Rg3 was 65.12%. Conclusion The method is simple in operation, low in cost with no pollution to environment, and is a feasible method for rapid preparation of 20 （ S ） -ginsenoside Rg3 and 20 （R） -ginsenoside Rg3.

关 键 词：人参皂苷Rg  谷氨酸 原人参二醇组皂苷 酸水解 正交试验 

分 类 号：R284[医药卫生—中药学]