黑豆皮中花青素大孔吸附树脂分离纯化工艺研究  

Study on the Process of Macroporous Resin Isolate and Purify Anthocyanin from Black Soybean Hull

作　　者：吴艳立 丁之恩[1] 闫晗[1] 董敏[1] 常慧[1] 亢金秀 

机构地区：[1]安徽农业大学,安徽合肥230036

出　　处：《食品研究与开发》2016年第21期50-53,67,共5页Food Research and Development

摘　　要：不同的大孔树脂对花青素的吸附能力不同,本试验依据花青素的特征,大孔树脂的特性,筛选出DM301、ADS-17、HPD-417 3种不同型号的大孔树脂,以吸附率α和解吸率β为评价指标,选择两种大孔树脂进行组合,对黑豆皮花青素粗提物进行吸附与解吸试验,采用UPLC-MS对纯化物进行检测。结果表明:最佳的树脂比例组合是DM301:HPD-417为4∶1(质量比),乙醇溶液体积分数65%,解吸液p H 3.5,动态吸附上样流速0.5 m L/min,洗脱液流速1.5 m L/min是最佳的选择,纯化的花青素主要成分为矢车菊素-3-O-葡萄糖苷。Different maeroporous resins have different abilities of adsorption for anthocyanin.According to the characteristics of anthocyanin and the characteristics of macroporous resin, three different types of macroporous （DM301,ADS-17,HPD-417） resins were selected in this experiment. ,the adsorption rate α and desorption rate [3 were act as evaluation index, two kinds of macroporous resins were selected for adsorption and desorption experiment of black soybean hull anthocyanin extracts. At last , use UPLC-MS to detect the purified anthocyanin. The results showed that The best resin ratio combination is 4 ： 1 for DM301 ：HPD-417 ,the ethanol volume fraction 65 %, the desorption liquid pH3.5, the dynamic adsorption on the sample flow rate 0.5 mL/min, the desorption flow rate 1.5 mL/min, the main component of the purified anthocyanins is Cyanidin-3-O-glucoside.

关 键 词：黑豆皮 提取 花青素 大孔树脂 组合 纯化 检测 

分 类 号：TQ461[化学工程—制药化工]