表没食子儿茶素没食子酸酯改性全酸蚀粘接剂对根管牙本质粘接界面稳定性的影响  

Effects of epigallocatechin-3-gallate modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin

作　　者：余昊翰[1] 张凌[1] 余凡[1] 周欢[1] 沈丽娟[1] 陈吉华[1] 

机构地区：[1]第四军医大学口腔医学院口腔修复科军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病重点实验室,西安710032

出　　处：《中华口腔医学杂志》2017年第2期114-119,共6页Chinese Journal of Stomatology

基　　金：国家自然科学基金（51373198、81130078、81571019）;教育部创新团队发展计划（IRT13051）

摘　　要：目的评价表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）改性粘接剂对根管牙本质粘接界面稳定性的作用，为提高粘接界面耐久性提供新策略。方法将EGCG混入全酸蚀粘接剂（Single Bond 2），制备质量浓度分别为200、400mg／L的EGCG改性粘接剂（改性A组和B组），以未添加EGCG的粘接剂（SingleBond2）为对照组。激光扫描共聚焦显微镜和扫描电镜观察粘接剂表面培养粪肠球菌24h后生物膜的生长情况；微拉曼光谱仪检测粘接剂双键转化率；收集30颗人无龋单根前磨牙，去除牙冠后用各组粘接剂分别制备纤维桩粘接试件，用于即刻（老化前）和老化后的微推出实验（每组每个时间点30个试件），测试粘接强度。结果改性粘接剂可抑制粪肠球菌生物膜形成。改性A和B组粘接剂双键转化率分别为（69．73±0．68）％和（69．03±1．65）％，与对照组[（70．06±1．62）％1差异无统计学意义（P〉0．05）。改性A和B组老化前粘接强度分别为（10．02±2．03）和（9．95±3．03）MPa，与对照组[（10．45±2．00）MPa]相比差异无统计学意义（P〉0．05）。老化后改性A和B组粘接强度[分别为（7．01±1．39）和（7．62±1．88）MPa]均显著高于对照组[（5．08±1．56）MPa]（P〈O．05）。结论EGCG改性后的全酸蚀粘接剂可抗粪肠球菌生物膜形成，并可提高根管牙本质粘接界面的稳定性。Objective To evaluate the effects of epigallocatechin-3-gallate （EGCG） modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin, and to find a new strategy to improve the stability of bonding interface. Methods EGCG was incorporated into Single Bond 2 （SB2） with the concentration of 200 mg/L and 400 mg/L respectively to fabricated experimental adhesives group A and group B, while Single Bond 2 without EGCG was used as control group. Laser scanning confocal microscope （LSCM） and scanning electron microscope （SEM） were used to observe the bacterial biofilm adherent to the surface of the cured adhesive. Micro-Raman spectrum was used to test the degree of conversion （DC） of adhesives. The push-out bond strength of instant testing and aging with thermocycling for 5 000 times were also tested. Results Group A and group B showed inhibiting effect on the biofilm formation of Enterococcus faecalis and performed better with higher concentration. No significant differences were detected in DC among group A （[69.73±0.68]%）, group B （[69.03± 1.651%） and control group （[70.06± 1.621%） （P〉0.05）, and the immediate push-out bond strength of control group （[10.45±2.00] MPa） was not compromised compared to group A （[10.02_＋ 2.03] MPa） and group B （[9.95 ＋ 3.03] MPa） （P〉0.05）. After thermocycling for 5 000 times, group A （[7.01 ± 1.39] MPa） and group B （[7.62 ± 1.88] MPa） showed significantly higher push-out bond strength than control group did （[5.08 ±1.56] MPa） （P〈0.05）. Conclusions EGCG modified etch-and-rinse adhesives showed anti-bacterial effect and enhanced bonding stability of intraradicular dentin-adhesive interfaces.

关 键 词：牙本质粘结剂 表没食子儿茶素没食子酸酯 抗菌 

分 类 号：R783.1[医药卫生—口腔医学]