木质纤维素原料氢氧化钠预处理及酶解特性研究  

Lignocellulose Materials Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis Characteristics Research

作　　者：陈婷婷[1] 何剑波[1] 凌宏志[1] 

机构地区：[1]黑龙江大学生命科学学院,微生物重点实验室,黑龙江哈尔滨150080

出　　处：《酿酒》2017年第1期71-75,共5页Liquor Making

摘　　要：木质纤维素组成结构十分复杂,没有经过处理的木质纤维素很难直接被酶解。预处理技术是木质纤维素降解的关键性技术。在我国,开发利用木质纤维素意义重大。对木质纤维素的预处理和水解。用玉米芯最为研究对象,优化了碱液预处理的条件,对预处理温度、时间和氢氧化钠浓度这三个因素对影响玉米芯各组分的分离效果及水解后产量的规律进行研究。研究结果显示,随着预处理反应的进行,在氢氧化钠处理液中,可溶性糖和木质素的量先急剧增加,后逐渐平稳。利用响应曲面法优化水解条件。采用了单因素多水平和正交试验的方法,最终得到了最优响应值的预处理条件为:反应时间2.14h、温度78.18℃和酸质量分数为2.21%,此时糖收率为56.67%,同时在试验设计的最佳水解条件下,进行的验证试验结果与模型预测值几乎相同,表明所设计的实验条件是合适的。Due to the special structure, untreated lignocellulosic was not able to be degraded directly enzyme. Pretreatment of lignocellulose degradation is a key technology. According to our national conditions, studying and exploitating cellulose resource have important sense.This thesis unfolds around lignocelluloses pretreatment and hydrolysis. Based on corn cobs, Alkali pretreatment conditions for lignocellulosic corn cob were optimized, The effects of reaction temperature, pretreatment time and concentration of Sodium hydroxide on the separated components and the obtained hydrolysates during the fractionation were studied. The results showed that the masses of soluble sugars and lignin increased quickly at first, and then changed slowly. The response surface methodology was used to optimize the hydrolysis conditions. By multilevel of single-factor test and orthogonal test methods, theresults for the optimal pretreatment conditions were as follows：reaction time 2.14 h, reaction temperature 78.18℃, Alkali mass fraction 2.21%. Under those conditions, sugar yield and mass concentration of furfural are 56.67%, The result conducted under the optimum conditions demonstrates that the models fit the experimental data well.

关 键 词：木质纤维素 氢氧化钠 预处理 酶解 

分 类 号：TS262.2[轻工技术与工程—发酵工程] TS261.41[轻工技术与工程—食品科学与工程]