丛枝菌根育苗基质对Cd胁迫下黄瓜幼苗生长及生理特性的影响  

Effect of Arbuscular Mycorrhizal on Growth and Physiology of Cucumber Seedlings Under Cd-stress

作　　者：吴晓蕾[1] 李潘[1] 张艳芳[2] 贾匆匆 武琳琳[1] 张宏僖 高洪波[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《福建农业学报》2016年第12期1323-1328,共6页Fujian Journal of Agricultural Sciences

基　　金：河北农业大学大学生创新训练计划项目(2015076);河北农业大学青年科学基金项目(QN201313)

摘　　要：采用基质栽培的方法,通过添加2个浓度(0.2、0.4mmol·L-1)的Cd2+,研究AM菌根育苗基质对Cd胁迫下黄瓜幼苗生长、光合及叶绿素荧光参数和活性氧代谢的影响。结果表明:在Cd胁迫下黄瓜幼苗的生长、光合作用以及光合系统活性均受显著抑制,而抗氧化酶活性显著提高,且表现为较高浓度的Cd胁迫下的幼苗生长和生理特性受抑制情况更严重;经菌根基质育苗的幼苗在不同浓度Cd胁迫下,均表现为叶面积、根系活力和地下干鲜重显著提高;叶片的Pn、Gs和Tr显著提高,Ci显著降低;叶片Fv/Fm、ETR、qP、фPSII显著提高,NPQ显著降低;POD、CAT等抗氧化酶活性进一步提高,而细胞MDA含量和O·-2产生速率显著降低。结果表明AM菌根可缓解黄瓜幼苗在Cd胁迫下所受到的生长抑制,并降低光合系统及细胞遭受的伤害,且对于较低浓度(0.2mmol·L-1)Cd胁迫的缓解作用更好。Effect of nursery substrates inoculated with arbuscular mycorrhizal on the growth and physiological characteristics of cucumber seedlings under Cd-stress was studied.Two levels of Cd,i.e.,0.2and 0.4mmol·L^-1,were artificially imposed on the substrate during the experimentation for observation.The results showed that the growth,photosynthesis and photosynthetic activity of the seedlings were inhibited,while the antioxidant enzymatic activities increased significantly under the Cd-stresses.The inhibitions were more pronounced with higher addition ofCd.On the other hand,with the aid of arbuscular mycorrhizal,the leaf area,root activity and underground dry weight of the seedlings cultivated under the Cd-stress increased dramatically.Meanwhile,Pn,Gs and Tr improved significantly,Ci decreased significantly,Fv/Fm,ETR,qP andфPSII improved significantly,and NPQ decreased significantly;while,the activities of POD and CAT were enhanced,and the O·-2 producing rate and MDA content decreased.It appeared that arbuscular mycorrhizal could alleviate the growth inhibition effects and decrease the damage to the photosynthetic system on the cucumber seedlings due to Cd-stress,especially,when the stress was less severe at a low level,such as 0.2mmol·L^-1.

关 键 词：黄瓜 丛枝菌根基质 CD胁迫 生长 光合作用 叶绿素荧光参数 活性氧代谢 

分 类 号：Q945.78[生物学—植物学] S642.2[农业科学—蔬菜学]