绞股蓝皂苷抑制HepG2细胞无氧糖酵解的检测分析  

Detection analysis of inhibition of anaerobic glycolysis by gypenosides in HepG2 cells

作　　者：文利[1] 郑新[1] 刘飞[2] 钱青[1] 

机构地区：[1]第三军医大学新桥医院药剂科,重庆400037 [2]第三军医大学新桥医院检验科,重庆400037

出　　处：《检验医学与临床》2017年第4期484-486,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨绞股蓝皂苷对肝癌细胞HepG2生长和无氧糖酵解的影响。方法在肝癌细胞HepG2培养基中加入不同浓度的绞股蓝皂苷,于0、4、8、16、24h分别收集培养基200μL。培养基葡萄糖和乳酸含量均采用生化流水线检测。结果 MTT数据表明绞股蓝皂苷明显抑制了肝癌细胞株HepG2的生长;浓度为5mg/mL时,抑制明显,且有利于后续无氧糖酵解分析。生化检测表明,绞股蓝皂苷显著降低了葡萄糖的消耗,两者差距最明显的时间点为6h(P<0.05);同时绞股蓝皂苷也明显降低了乳酸的生成(P<0.05)。结论绞股蓝皂苷显著抑制HepG2无氧糖酵解,为绞股蓝在肝癌治疗的临床用药提供了一定的线索和依据。Objective The research aims to explore gypenoside influence on the growth and anaerobic glycolysis in HepG2 cell.Methods Gypenosides with different concentrations were added into culture medium of HepG2 cell,and 200μL of cell cuture medium were collected at 0,4,8,16,24 htime.The concentration of medium glucose and lactic acid were detected by biochemical detection pipeline.Results The test data show that gypenoside significantly inhibited the growth of HepG2cells;where the concentration of 5mg/mL is the proper choice of the inhibition and favor of anaerobic glycolysis analysis.Follow-up testing found gypenosides significantly inhibited glucose consumption,with the difference is most obvious at a time point 6h（P〈0.05）.Meanwhile,gypenoside significantly reduced lactate production（P〈0.05）.Conclusion Experimental results show that gypenoside significantly inhibited anaerobic glycolysis of HepG2 cell,which provides some clues for gynostemmaclinical use in cancer treatment.

关 键 词：绞股蓝皂苷 HEPG2 无氧糖酵解 葡萄糖 乳酸 

分 类 号：R735.7[医药卫生—肿瘤]