顶空固相微萃取-气相色谱-质谱联用分析柚木光辐射前后的挥发性成分  

Analysis of Volatile Constituents in Tectona grandis L. F. Before and After Irradiation by HS-SPME-GC-MS

作　　者：文瑞芝[1] 曾栋[2] 黄自知[1] 马强[1] 王琼[1] 胡云楚[1] 

机构地区：[1]中南林业科技大学理学院,湖南长沙410004 [2]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《林产化学与工业》2017年第1期135-140,共6页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(31170521);湖南省教育厅项目(14C1188)

摘　　要：采用顶空固相微萃取-气相色谱-质谱联用技术检测分析了柚木光辐射前后的挥发性成分,研究结果表明:从光辐射前后的柚木中共鉴定出80种挥发性成分,辐射前(原柚木)鉴定出57种挥发性成分,辐射60 h后的柚木中鉴定出53种挥发性成分,包括烃类、醇类、酚类、酯类、醛类、酮类、蒽醌类和胺类。光辐射后柚木未能检测到酮类,且醇类从12.586%减少到7.498%,醇类化合物由6种减少至3种,醛类从0.404%增加到3.653%,醛类化合物由3种增至5种。柚木光辐射前挥发性成分主要是2-甲基蒽醌(18.443%)、2-N-甲基-萘胺(14.781%)、邻苯二甲酸异丁基辛基酯(9.567%)、1,2,3-三甲基-4-丙烯基-(E)-萘(7.513%)、τ-杜松醇(4.684%)、α-杜松醇(3.583%)、柏木脑(3.576%)、1,2-邻苯二甲酸丁基异丁基酯(3.213%)这8种化合物;辐照后除α-杜松醇和1,2-邻苯二甲酸丁基异丁基酯未能检测到外,增加了化合物1,2-邻苯二甲酸丁基环己基酯(3.070%),且邻苯二甲酸异丁基辛基酯(11.787%)有所增加,其余成分含量变化不大。醛类挥发性成分含有发色基团—CHO,醇类挥发性成分含有助色基团羟基—OH,而且光辐射后含量明显增加的挥发性成分都含有发色基团—COO—,这些发色基团和助色基团的改变在柚木光变色中起到一定作用。Headspace solid-phase micro-extraction（HS-SPME） combined with gas chromatography-mass spectrometry（GC-MS） technology was used to analyze the volatile constituents in original and irradiated Tectona grandis L.F.. The results showed that 80 kinds of volatile constituents were identified. There were 57 volatile constituents in T. grandis without irradiation and 53 volatile constituents in T. grandis after irradiation. They included hydrocarbons, alcohols, phenols, esters, aldehydes, ketone, anthraquinone and amine. After irradiation, the ketone in the volatile constituents disappeared, the content of alcohols decreased to 7.498% from 12.586% and the species decreased to 3 from 6, and the aldehydes increased to 3.653% from 0.404% with the species of 5 from 3. The major volatile constituents before irradiation were 2-methyl-anthracenedione（18.443%）, 2-N-methyl-naphthalenamine（14.781%）, phthalic acid-isobutyl octyl ester（9.567%）, 1,2,3-trimethyl-4-propenyl-（E）-naphthalene（7.513%）, τ-cadinol（4.684%）, α-cadinol（3.583%）, cedrol（3.576%） and 1,2-benzenedicarboxylic acid-butyl 2-methylpropyl ester（3.213%）, respectively. After irradiation, the α-cadinol and 1,2-benzenedicarboxylic acid-butyl 2-methylpropyl ester disappeared, the 1,2-benzenedicarboxylic acid-butyl cyclohexyl ester appeared with the content of 3.070%, the content of phthalic acid-isobutyl octyl ester increased by 2.22 percentage points, and the contents of others changed little. The alcohol volatile components might be oxidized to the aldehydes volatile components by irradiation. The aldehydes contains the chromophoric group of -CHO, and the alcohols contains the auxochrome group of -OH, and the significant increase of the constituents after irradiation contains the chromophoric group of -COO-. The hydroxyl, aldehyde group and ester group might play an important role in the T.grandis photo-discoloration, but to well understand the the mechanism of photo-discoloration, further studies were needed.

关 键 词：柚木 挥发性成分 光辐射 顶空固相微萃取 

分 类 号：TQ35[化学工程]