阳性血培养瓶里“失踪”的肺炎链球菌  

The disappeared Streptococcus pneumoniae in positive blood culture flask

作　　者：周世娟 

机构地区：[1]广东省惠东县人民医院,广东惠东516300

出　　处：《中国当代医药》2017年第3期126-128,共3页China Modern Medicine

基　　金：广东省惠州市科技计划项目(20160806)

摘　　要：目的探讨如何鉴定阳性血培养瓶里"失踪"的肺炎链球菌(Streptococcus pneumoniae,S.pn)。方法采用靶向DNA测序(16S r RNA基因扩增)的方法最后鉴定出"失踪"的细菌。结果 "失踪"的细菌被鉴定为肺炎链球菌。结论依据靶向DNA测序(16S r RNA基因扩增)鉴定可及时、准确鉴定出因自溶死亡的肺炎链球菌,启示微生物检验程序中血培养报阳后应立即涂片、转种平板,并结合血培养生长曲线、涂片染色结果作进一步的分析等,以提高血培养的阳性检出率,有利于减少漏报及误报的发生。Objective To explore how to identify the disappeared Streptococcus pneumoniae in positive blood culture flask.Methods By targeted DNA sequencing （16S rRNA gene amplification）,the disappeared bacterium was finally identified.Results The disappeared bacterium was identified as Streptococcus pneumoniae.Conclusion By targeted DNA sequencing （16S rRNA gene amplification） identification can detect the Streptococcus pneumoniae dead due to autolysis.It is indicated that in the process of bacteriological examination,smear and transmission to plate combined with growth curve and smear results of blood culture are performed right away after blood culture being positive.Together with growth curve of blood culture and outcomes of smear staining,further analysis is needed in order to increase positive detection rate of blood culture and reduce occurrence of report in fail and mistake.

关 键 词：16S RRNA 测序 肺炎链球菌 自溶 

分 类 号：R446.5[医药卫生—诊断学]