温胆颗粒中挥发性成分的HS-SPME-GC/MS分析  

HS-SPME-GC/MS analysis of volatile components in Yi-Zhi-Wen-Dan granules

作　　者：刘军锋[1] 昝俊峰[2] 王平[3] 游秋云[2] 方颖[1] 田代志[3] 张丽萍[1] 呙霞 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉430065 [2]湖北中医药大学药学院,武汉430065 [3]湖北中医药大学老年医学研究所,武汉430065

出　　处：《时珍国医国药》2017年第1期67-70,共4页Lishizhen Medicine and Materia Medica Research

基　　金：高等学校博士学科点专项科研基金项目(No.20124230120004);国家自然科学基金重点项目(No.81130064)

摘　　要：目的以院内制剂温胆颗粒为研究对象,建立快速、简便、环保、样品用量少、便于实现自动化和易与色谱分析技术联用的中药复方制剂挥发性成分分析方法。方法运用顶空固相微萃取技术富集温胆颗粒中的挥发性成分,并运用气相色谱-质谱联用分析技术结合NIST谱库检索分析其成分类别及相对含量。结果共检测出163种挥发性的成分,鉴定出74个色谱峰,占检出挥发性成分总含量的96.35%。其中主要化学成分是芳樟醇、丁香酚甲醚、异丁香酚甲醚、β-细辛醚、欧细辛醚、桔利酮、丁烯基苯酞、洋川芎内酯A、反式藁本内酯等,结合文献确定其主要来源为复方中的生姜、石菖蒲、川芎等药材。结论运用HS-SPME-GC/MS技术可快速检测温胆颗粒中的挥发性成分,并可应用于相关复方挥发性成分的血清药化学和脑组织液药化学研究工作。Objective Wen-Dan granule（ Modified Wendan Decoction,MWD） is a hospital preparation for the treatment of Alzheimer’s disease. It is a modern dosage form produced based on a modified prescription of Wendan Decoction,which was an ancient and classical prescription with the function of＂Hua-Tan＂. In this paper,in order to establish a rapid,convenient,environmental friendly,low sample consumption analysis technology for traditional Chinese medicine volatile components analysis.Methods Headspace solid phase micro-extraction（ HS-SPME） was applied for the enrichment of volatile components of MWD,followed by gas chromatography-mass spectrometry（ GC/MS） analysis,and then the mass spectrometric data were analyzed based on NIST spectrum library. Results In total,163 volatiles were isolated and 73 components were determined,accounting for 96. 35% of the total content of all volatile components. The main chemical compositions were Linalool,Methyl eugenol,Methyl isoeugenol,β-Asarone,Euasarone,Zierone,Butylidene phthalide,Senkyunolide A and Trans-ligustilide,etc. Conclusion According to the related literatures,the possible sources of these compounds were herbs in MWD prescription that contain large amount of volatile oil,such as Zingiber officinale Rosc.,Acorus tatarinowii and Ligusticum chuanxiong Hort.,etc. The application of the HS-SPME-GC/MS technique can detect volatile components of MWD efficiently,and it may be applied for the analysis of volatile components in drug-containing serum and brain tissue fluid.

关 键 词：化学计量学解析法 温胆颗粒 顶空固相微萃取 气相色谱-质谱 

分 类 号：R284.1[医药卫生—中药学]