caveolae介导的apelin-13促内皮细胞增殖机制探讨  

Search for the mechanism of caveolin by caveolin-1 biomarker protein on endothelial cells proliferation which induced apelin-13

作　　者：李芳[1] 潘红宁[1] 王苏平[1] 朱慧[1] 吴华平[1] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224005

出　　处：《贵州医药》2017年第1期17-19,F0003,共4页Guizhou Medical Journal

基　　金：盐城市卫生局科技计划(项目编号:YK2012036)

摘　　要：目的探讨caveolae及其标志蛋白caveolin-1在apelin-13促内皮细胞增殖中的作用,并对apelin-13促内皮细胞增殖信号转导通路进行拓展性研究。方法 MTT比色法观察caveolae在apelin-13促内皮细胞增殖中的作用;Western Blot检测不同因素处理下,内皮细胞中信号蛋白的表达;免疫共沉淀技术检测不同因素处理后,内皮细胞中信号分子复合物的形成情况。结果β-环糊精作为caveolae结构破坏剂(5mmol/L,24h)对apelin-13诱导的内皮细胞增殖具有明显的增强作用;apelin-13处理内皮细胞后,caveolin-1的表达减少,且它的减少在2μmol/L时表现尤为明显;内皮细胞在使用β-环糊精(5mmol/L)预孵育后,apelin-13下调caveolin-1表达的作用增强;对照组及apelin-13(2μmol/L)处理组caveolin-1与PI3K、ERK1/2均有复合物形成,apelin-13处理组较对照组caveolin-1-PI3K及caveolin-1-ERK1/2两种复合物的形成均有所减少。结论 caveolae及其标志性蛋白caveolin-1参与apelin-13促内皮细胞增殖过程;apelin-13促内皮细胞增殖作用可能与促进caveolin-1与PI3K、ERK1/2解离有关。ObjectiveTo discuss the effect of caveolae and caveolin-1 on endothelial cells proliferation which in- duced by apelin-13, and make an expansive study on signal transduction pathway of apelin-13 induced endothelial cells proliferation in the meanwhile. Methods The effect of i3-cyclodextrin （13-CD） on apelin-13 induced endothelial cells proliferation was measured by MTT assay. After treating with different factors, the expression of caveolin-1 in endothelial cells was detected by western blot. Formation of caveolin-l-PI3K and caveolin-l-ERK1/2 complexes was detected by co-immunoprecipitation. Results β-CD （5mmol/L, 24 h） significantly enhanced endothelial cells prolifer- ation induced by apelin-13. After treating with apelin-13, the expression of caveolin-1 in endothelial cells decrease and the effect was most significant at 2umol/L. Pretreating endothelial cells with 5mmol/L β-CD could strength the downregulation of apelin-13-induced caveolin-1 expression. Apelin-13 may induce dissociation of caveolin-l-PI3K and caveolin-l-ERK1/2 complex in endothelial cells compared with the control group. Conclusion These findings suggest that caveolae and caveolin-1 are involvedin apelin-13-induced endothelial cells proliferation.

关 键 词：内皮细胞 APELIN-13 CAVEOLAE CAVEOLIN-1 PI3K ERK1/2 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]