Synthesis of aptamer-functionalized Ag nanoclusters for MCF-7 breast cancer cells imaging  

作　　者：Taotao Li Jingjing Yang Zeeshan Ali Zunliang Wang Xianbo Mou Nongyue He Zhifei Wang 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]School of Chemistry and Chemical Engineering,Southeast University,Nanjing 210096,China

出　　处：《Science China Chemistry》2017年第3期370-376,共7页中国科学（化学英文版）

基　　金：supported by the National Basic Research Program of China(2014CB744501);the National Natural Science Foundation of China(61271056,61527806);the Natural Science Foundation of Jiangsu Province(BK20141332);the National Youth Science Foundation of China(61301043);the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Fundamental Research Funds for the Central Universities

摘　　要：Aptamer-functionalized silver nanoclusters(Ag NCs) have been attracting a lot of interest as label-free probes which have been successfully applied to both cell imaging and molecular detection.MUCl aptamer is an ssDNA aptamer that specifically binds to MUCl mucin which is a large transmembrane glycoprotein whose expression level increases at least 10-fold in primary and metastatic breast cancers.Using C4A4C3-linker-MUCl as template,the Ag NCs were synthesized through one-pot process.The fluorescence intensity of Ag NCs was found to be closely related to the length and type(poly adenine or thymine) of the linker,the optimum linker being-AAAAA-.Using the C4A4C3-A5-MUC1 as the scaffold,the synthesized Ag NCs emitted fluorescence with high quantum yield(QY) of 66.5%.Based on the specific interaction between the MUCl aptamer and MUCl mucin,the C4A4C3-A5-MUC1-stabilized Ag NCs could recognize and differentiate the MCF-7 breast cancer cells from MDA-MB-231 breast cancer and A549 human lung cancer cells.Aptamer-functionalized silver nanoclusters（Ag NCs） have been attracting a lot of interest as label-free probes which have been successfully applied to both cell imaging and molecular detection.MUCl aptamer is an ssDNA aptamer that specifically binds to MUCl mucin which is a large transmembrane glycoprotein whose expression level increases at least 10-fold in primary and metastatic breast cancers.Using C4A4C3-linker-MUCl as template,the Ag NCs were synthesized through one-pot process.The fluorescence intensity of Ag NCs was found to be closely related to the length and type（poly adenine or thymine） of the linker,the optimum linker being-AAAAA-.Using the C4A4C3-A5-MUC1 as the scaffold,the synthesized Ag NCs emitted fluorescence with high quantum yield（QY） of 66.5%.Based on the specific interaction between the MUCl aptamer and MUCl mucin,the C4A4C3-A5-MUC1-stabilized Ag NCs could recognize and differentiate the MCF-7 breast cancer cells from MDA-MB-231 breast cancer and A549 human lung cancer cells.

关 键 词：MCF-7 cells cell typing Ag NCs APTAMER one-pot process 

分 类 号：R73-3[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]