分子生物学技术在肠道微生物研究中的应用进展  

Application of molecular biotechnology on gut microecology:Research progress

作　　者：杨翠[1] 李祖明[1] 

机构地区：[1]北京联合大学应用文理学院,北京100191

出　　处：《中国微生态学杂志》2017年第2期229-233,238,共6页Chinese Journal of Microecology

基　　金：北京市教委面上项目(KM201311417007);北京市教育委员会科技成果转化和产业化项目(PXM2013_014209_07-000082)

摘　　要：肠道微生物与宿主的健康状况息息相关,已成为当今的热点研究领域。随着分子生物学技术的快速发展,高通量测序技术、实时荧光定量PCR技术和PCR-DGGE技术等凭借其高灵敏度、高通量、无需体外培养等优势,为研究微生物结构和功能基因组提供了新方法,并在肠道菌群的研究中应用广泛。本文对这三种分子生物学技术在肠道微生物研究中的应用进行了综述,总结了这三种技术在肠道微生物研究中的应用范围和优缺点,并展望了其在肠道微生物研究中的广阔应用前景。Gut microbiota is closely related to the health of the host,which has received significant attention from researchers.With the development of molecular biology technologies,high-throughput sequencing technology,quantitative real-time PCR and PCR-DGGE,which have the advantages of high sensitivity,high throughput and no need for in vitro culture,new methods for studying the structure and functional genomics of microbes become available,and these methods have been extensively applied in the researches on gut microbiota.In this paper,the applications of molecular biology technologies on gut microbiota were reviewed,including high-throughput sequencing technology,quantitative real-time PCR and PCR-DGGE.The application range,advantages and disadvantages of the three techniques for the researches of gut microbiota were summarized respectively,and the prospects of their application in the researches of gut microbiota were discussed.

关 键 词：肠道微生物 高通量测序 实时荧光定量PCR 变性梯度凝胶电泳 

分 类 号：Q938.1[生物学—微生物学]