肝细胞癌中Dkk-3与CyclinD1的表达及临床意义  

Expression of Dkk-3 and Cyclin D1 protein in hepatocellular carcinoma and their clinical significance

作　　者：姚楠[1] 孙景秋[2] 张琼[1] 

机构地区：[1]安徽省蚌埠医学院第一附属医院病理科,蚌埠233003 [2]安徽省蚌埠医学院第一附属医院核医学科,蚌埠233003

出　　处：《临床与实验病理学杂志》2017年第2期158-161,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：蚌埠医学院校级课题基金(Byky1316)

摘　　要：目的探讨Dkk-3与Cyclin D1蛋白在肝细胞癌(hepatocellular carcinoma,HCC)和癌旁组织中的表达,及两者表达与临床病理特征的相关性。方法采用免疫组化法检测80例HCC及癌旁组织中Dkk-3和Cyclin D1蛋白的表达。结果 Dkk-3蛋白在HCC组织中的相对表达值低于癌旁组织(P<0.05);Cyclin D1蛋白在HCC组织中的相对表达值高于癌旁组织(P<0.005);Dkk-3和Cyclin D1蛋白相对表达值均与组织分化程度有关(P均<0.05);HCC组织中Dkk-3与Cyclin D1蛋白表达呈负相关(P=0.044,rs=-0.226)。结论 Dkk-3蛋白可能通过影响Cyclin D1蛋白的表达影响HCC的发生、发展;联合检测Dkk-3和Cyclin D1蛋白表达可能对HCC的诊断及预后判断有一定参考价值。Purpose To investigate the expression of Dkk- 3 and Cyclin D1 protein in human hepatocellular carcinoma （ HCC ） and clinical significance. Methods Immunohistochemistry was used to detect Dkk-3 and Cyclin D1 protein expression level in 80 cases of hepatocellular carcinoma and corresponding para-cancer tissue. Results The expression of Dkk-3 in hepatocellular carcinoma was significantly lower than those in corresponding para-cancer tissue （ P 〈 0. 05 ） and the expression of Cyclin D1 in hepatocellular carcinoma was significantly higher than those in corresponding para-cancer tissue （ P 〈 0. 05）. The up-regulation of Cyclin D1 and the down-regulation of Dkk-3 proteins were correlated with pathologic differentiation degree （P 〈 0. 05）. There was a significant inverse correlation between Dkk- 3 and Cyclin D1 expression （P = 0. 044, rs = - 0. 226）. Conclusion The abnormal expression of Dkk-3 and Cyclin D1 gene in human hepatocellular carcinoma suggest that Dkk-3 and Cyelin D1 gene may play an important role in the development and progression of the cancer. The combination detection of the two biomarkers may provide valuable data for diagnosis and prognosis estimation of HCC.

关 键 词：肝肿瘤 Dkk-3 CYCLIN D1 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]