七元瓜环对2＇-羟基查尔酮溶解性、稳定性及抗氧化活性的影响  

Influence of Interaction with Cucurbit [7] uril on Solubility,Stability and Antioxidant Activity of 2'-Hydroxychalcone

作　　者：李梦杰[1] 练小卫 许志玲[2] 邢旭阳 张建新[3] 李瑞[1] 陶朱[1] 张前军[1] 

机构地区：[1]贵州省大环化学及超分子化学重点实验室,贵阳550025 [2]贵州大学化学与化工学院精细化工中心,贵阳550025 [3]中国科学院贵州省天然产物化学重点实验室,贵阳550025

出　　处：《高等学校化学学报》2017年第3期442-447,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21272045)资助~~

摘　　要：利用~1H NMR、紫外吸收光谱和荧光光谱等方法考察了七元瓜环(Q[7])对2'-羟基查尔酮(CET)的包结作用.结果表明,Q[7]与CET形成了摩尔比为1∶1的包结配合物,紫外吸收光谱和荧光光谱测得的结合稳定常数分别为1.0248×10~6和1.253×10~6.相溶解度法研究结果表明,当Q[7]的浓度为1×10^(-3)mol/L时,可使CET在水中的溶解度增加52倍.紫外吸收光谱随时间变化的研究结果表明,Q[7]使CET的稳定性增加3.5倍.采用体外抗氧化活性测定(ABTS法)考察了Q[7]对CET抗氧化活性的影响,发现CET@Q[7]包结配合物以及游离CET均对ABTS自由基有较好的清除作用.IC50值分别为3.4×10^(-5)和2.4×10^(-5)mol/L,表明Q[7]不仅能增加CET的溶解性和稳定性,同时对CET抗氧化活性的影响不大.The host-guest interactions and properties of 2＇-hydroxychalcone （CET） with cucurbit [ 7 ] uril （Q [ 7 ] ） were investigated by means of 1H NMR, IR, UV-Vis absorption and fluorometry spectroscopy. The results revealed that 2＇-hydroxyehalcone could form an inclusion complex of 1 ： 1 with Q [ 7 ]. The inclusion constants of CET with Q[ 7] obtained by UV absorption and fluorescence spectroscopy were 1. 0248×10^-6 and 1. 253×10^-6, respectively. The phase-solubility experiments revealed that the solubility of CET increased 52 times by interacting with Q [ 7 ] [ c （ Q [ 7 ] ） = 1 ×10^-3 mol/L ]. The time-scaled UV absorption spectra showed that Q [ 7 ] increases the stability of CET by 3.5 times. The antioxidant activity （ ABTS method） of both the CET Q[ 7] inclusion complex and CET proved to be good scavenging effect to the ABTS radical, IC50 values were 3.4×10^-5 and 2. 4×10^-5 mol/L, respectively. Thus, Q [ 7 ] could not only increase the solubility and stability of CET, but also have little effect on the antioxidant activity of CET. As a result, stability and water solubility were obviously improved.

关 键 词：查尔酮 ) 包结配合物 抗氧化活性 稳定性 

分 类 号：O641.3[理学—物理化学]