大鼠死后肌肉电导率与腐败程度的关系  

Relationship between Electrical Conductivity and Decomposition Rate of Rat Post-mortem Skeletal Muscle

作　　者：夏志远[1] 翟仙敦[1] 刘蓓蓓[1] 郑哲[1] 赵琳琳[1] 莫耀南[1] 

机构地区：[1]河南科技大学法医学院,河南洛阳471003

出　　处：《法医学杂志》2017年第1期17-20,共4页Journal of Forensic Medicine

基　　金：河南省基础与前沿课题资助项目(112300410082)

摘　　要：目的分析大鼠死后肌肉电导率(electrical conductivity,EC)、肉类食品腐败程度指标挥发性盐基氮(total volatile basic nitrogen,TVB-N)与死亡时间(postmortem interval,PMI)的关系,探讨EC作为尸体肌肉腐败程度评价指标的可行性,为该法用于PMI推断奠定理论基础。方法健康SD大鼠颈椎脱臼处死后,保存在28℃环境中,于死后不同时间点提取大鼠后肢肌肉组织,用去离子水制成质量浓度为0.1 g/m L的肌肉浸渍液,并测量所得浸渍液的EC值和TVB-N含量。分析EC(x_1)与TVB-N(x_2)的相关性,建立二者关系的回归方程;分析两指标与PMI的相关性,并分别建立两指标与PMI(y)关系的回归方程。结果肌肉浸渍液EC、TVB-N随PMI的变化曲线走势基本相同;EC与TVB-N呈显著直线正相关,回归方程为x_2=0.14 x1-164.91(R^2=0.982);EC、TVB-N与PMI显著相关,回归方程分别为y=19.38 x_1~3-370.68 x_1~2+2 526.03 x1-717.06(R^2=0.994),y=2.56 x_2~3-48.39 x_2~2+330.60 x_2-255.04(R2=0.997)。结论大鼠死后肌肉EC与TVB-N变化趋势一致,可以作为反映尸体肌肉腐败程度的指标,为腐败尸体PMI推断的相关研究提供方法。Objective To analyze the relationship among electrical conductivity（EC）, total volatile basic nitrogen（TVB-N）, which is an index of decomposition rate for meat production, and postmortem interval（PMI）. To explore the feasibility of EC as an index of cadaveric skeletal muscle decomposition rate and lay the foundation for PMI estimation. Methods Healthy Sprague-Dawley rats were sacrificed by cervical vertebrae dislocation and kept at 28 ℃. Muscle of rear limbs was removed at different PMI, homogenized in deionized water and then skeletal extraction liquid of mass concentration 0.1 g/mL was prepared. EC and TVB-N of extraction liquid were separately determined. The correlation between EC（x1） and TVB-N（x2） was analyzed, and their regression function was established. The relationship between PMI（y） and these two parameters were studied, and their regression functions were separately established. Results The change trends of EC and TVB-N of skeletal extraction liquid at different PMI were almost the same, and there was a linear positive correlation between them. The regression equation was x2=0.14 x1-164.91（R2=0.982）. EC and TVB-N of skeletal muscle changed significantly with PMI, and the regression functions were y=19.38 x1-3-370.68 x1^2＋2 526.03 x1-717.06（R^2=0.994）, and y=2.56 x2^3-48.39 x2^2＋330.60 x2-255.04（R^2=0.997）, respectively. Conclusion EC and TVB-N of rat postmortem skeletal muscle show similar change trends, which can be used as an index for decomposition rate of cadaveric skeletal muscle and provide a method for further study of late PMI estimation.

关 键 词：法医病理学 腐败程度 肌 骨骼 电导率 挥发性盐基氮 死亡时间 大鼠 

分 类 号：R155.55[医药卫生—营养与食品卫生学]