基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚糖单糖组成分析  

Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

作　　者：刘晓莹[1] 马璐瑶[1] 王力[1] 汪星逸[1] 聂少平[1] 谢明勇[1] 殷军艺[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《分析化学》2017年第3期416-422,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.31301434;21564007);江西省优势科技创新团队(No.20133BCB24001);江西省自然科学基金(No.20142BAB214004)资助~~

摘　　要：以大粒车前子来源阿拉伯木聚糖为研究对象,采用完全酸水解结合高效阴离子色谱-脉冲安培法测定单糖组成,系统优化酸的种类和浓度、水解温度和时间、水解后样品放置时间等水解条件。结果表明,将车前子多糖置于2 mol/L H_2SO_4、120℃常压油浴条件下水解2 h时,效果较好,但水解后稀释液放置时间不宜超过6 h。大粒车前子多糖主要由阿拉伯糖(8.89%)和木糖(41.52%)组成,同时检测出半乳糖醛酸(0.73%)、葡萄糖醛酸(3.44%)和微量的半乳糖、葡萄糖,结果重现性良好。利用上述最优条件水解黍子壳、燕麦麸皮和青稞来源阿拉伯木聚糖并分析单糖组成,均获得较好结果。本研究为分析各种来源阿拉伯木聚糖的单糖组成提供了参考。A method of complete acid hydrolysis combined with high performance anion exchange chromatography and pulsed amperometric detection was developed for the monosaccharide composition analysis of arabinoxylan from the seeds of plantago asiatica L. The parameters including hydrolysis methods, acid types, acid concentration, hydrolysis temperature, hydrolysis time and placement time, which would affect the hydrolysis process, were optimized. The results showed that it would have a better hydrolysis effect for polysaccharide from the seeds of Plantago asiatica L. with 2 mol/L H2SO4 in an atmospheric oil bath at 120℃ for 2 hours. However, the placement time for diluted solution of the hydrolyzed polysaccharide should be less than 6 hours. The polysaccharide was mainly composed of Arabinose （8.89%） and Xylose （41.52%） and Galacturonic acid （0.73%）. Glcuronic acid （3.44%） was detected simultaneously, and there were also trace amounts of Galatose and Glucose. The results were reproducible. Other arabinoxylans from Panicummiliaceum L. shell, Avena sativa L. bran and Hordeum vulgare L. were taken for monosaccharide compositions analysis under the optimal hydrolysis conditions and the analysis results were good. This study would provide a good reference for monosaccharides composition analysis of arabinoxylans from various sources.

关 键 词：阿拉伯木聚糖 大粒车前子 高效阴离子色谱 水解条件 单糖组成 

分 类 号：O636.1[理学—高分子化学] O657[理学—化学]