米曲霉M-4降解己烯雌酚的条件优化  

Optimization of diethylstilbestrol degradation by Aspergillus oryzae M-4

作　　者：胡凯弟 柴先杜 陈树平[1] 邓维琴 周康[1,2] 刘书亮[1,2] 

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]四川农业大学食品加工与安全研究所,四川雅安625014

出　　处：《微生物学通报》2017年第3期525-532,共8页Microbiology China

基　　金：四川省农业科技成果转化资金项目(No.14NZ0012)~~

摘　　要：【目的】以米曲霉(Aspergillus oryzae)M-4对己烯雌酚(Diethylstilbestrol,DES)的降解率为响应值,对其降解条件进行优化。【方法】采用Plackett-Burman法对培养基组分和降解条件筛选显著性影响因素,并通过Box-Bohnken设计试验优化降解条件。【结果】最优培养基配方为:蛋白胨1.3%,CaCl_2 0.045%,葡萄糖0.5%,K_2HPO_4 0.15%,KH_2PO_4 0.05%,NaCl 0.05%,Tween 80 0.2%,DES质量浓度44 mg/L;最优培养条件为:初始p H 7.5,种龄72 h,转速140 r/min,培养温度28°C,培养时间72 h。【结论】在最优条件下菌株M-4对DES降解率为83.89%,比优化前(60.98%)提高1.38倍,差异极显著(P<0.01)。[Objective] We optimized degradation conditions for diethylstilbestrol（DES） by Aspergillus oryzae M-4. [Methods] Medium components and culture conditions were screened by using Plackett-Burman method. The degradation conditions were then optimized through Box-Bohnken design. [Results] The optimal medium was composed of 1.3% peptone, 0.045% CaCl2, 0.5% glucose, 0.15% K2HPO4, 0.05% KH2PO4, 0.05% NaCl, 0.2% Tween 80 and 44 mg/L diethylstilbestrol. The optimal culture conditions were as follows： initial pH 7.5, inoculum age 72 h, rotation rate 140 r/min, culture temperature 28 ℃ culture time 72 h. [Conclusion] Under the optimal conditions, the degradation rate of diethylstilbestrol was 83.89%, 1.38-fold over the value before optimization（60.98%）, showing a significant difference（P〈0.01）.

关 键 词：己烯雌酚 米曲霉 降解条件 优化 

分 类 号：X592[环境科学与工程—环境工程] X172