抗缺氧功能发酵物的筛选及其对缺氧大鼠糖代谢关键酶活性的影响  

Screening of Anti-Hypoxia Functional Fermented Broth and Their Effects on the Activity of Glycometabolism Key Enzymes in Hypoxia Rats

作　　者：吴天一 祁生贵 郝利民 郑志强 余坚勇 鲁吉珂[3] 

机构地区：[1]青海高原医学科学研究院,810012 [2]军委后勤保障部军需装备研究所,100010 [3]郑州大学生命科学学院,450001

出　　处：《高原医学杂志》2016年第4期1-5,共5页Journal of High Altitude Medicine

基　　金：国家自然基金裂褶菌发酵物抗缺氧机理研究(编号:31171662)

摘　　要：目的:研究裂褶菌发酵物对模拟缺氧大鼠糖代谢关键酶活性的影响。方法:采用小鼠密闭缺氧存活实验筛选抗缺氧功能发酵物,考察裂褶菌发酵物对大鼠神经元细胞缺氧缺糖损伤保护作用,并研究其对缺氧大鼠骨骼肌(腓肠肌)、左心室和右心室心肌的琥珀酸脱氢酶、乳酸脱氢酶活性的影响。结果:裂褶菌发酵物具有显著的抗缺氧功能,在一定浓度范围内可显著提高缺氧缺糖神经元细胞的存活率。裂褶菌发酵物可显著提高左心室琥珀酸脱氢酶活性,同时对右心室的琥珀酸脱氢酶活性具有一定的影响。结论:裂褶菌发酵物的抗缺氧机制与提高心肌琥珀酸脱氢酶活性有关。Objective：To investigate the effect of Schizophyllum commune fermented broth（SCFB）on the activity of glycometabolism key enzymes in hypoxia rats.Methods：Anti-hypoxia functional fermented broth were screened by acute hypoxic experiment with mouse.Then the protective effect of SCFB on neurons after oxygen-glucose deprivation in the rats were investigated,followed by the study of the effect on the activity of succinate dehydrogenase（SDH）and lactate dehydrogenase（LDH）from skeletal muscle and cardiac muscle of hypoxia rats.Results：SCFB showed remarkable anti-hypoxia function,and it could increase the survival rate of neurons after oxygen-glucose deprivation within a certain concentration range.SCFB could significantly improve the activity of the left ventricular SDH,and it also had effect on the activity of SDH in the right ventricle.Conclusion：The anti-hypoxia mechanism of SCFB is related to the increase of the SDH activity in myocardium.

关 键 词：裂褶菌 发酵物 缺氧 琥珀酸脱氢酶 乳酸脱氢酶 

分 类 号：R151[医药卫生—营养与食品卫生学]