不同诱导条件对茶树体胚诱导及增殖的影响  

The Effect on Embryogenic Induction and Proliferation of Camellia sinensis in Different Inductive Condition

作　　者：田丽丽[1,2,3] 李娟[1,2] 张静[1,2] 刘建军[1,2,4] 王坤波[1,2] 黄建安[1,2] 刘仲华[1,2] Tian Lili Li Juan Zhang Jing Liu Jianjun Wang Kunbo Huang Jian＇an Liu Zhonghua(Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, 410128 National Research Center of Engineer- ing Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, 410128 Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Tai＇an, 271000 College of Horticulture, He＇ nan Agricultural University, Zhengzhou, 450002)

机构地区：[1]湖南农业大学茶学教育部重点实验室、湖南农业大学,长沙410128 [2]国家植物功能成分利用工程技术研究中心、湖南农业大学,长沙410128 [3]山东省果树研究所,泰安271000 [4]河南农业大学,园艺学院,郑州450002

出　　处：《分子植物育种》2017年第2期669-671,共3页Molecular Plant Breeding

基　　金：国家茶叶产业技术体系项目(CARS-23-11B);国家自然科学基金项目(31470692;31500567;31670691)共同资助

摘　　要：为了获得茶树的体胚,本研究以福鼎大白、碧香早和白毫早的自然杂交实生种子为外植体,进行组织培养及植株再生研究。通过优化不同浓度的植物生长调节剂组合以及不同培养基和不同光照的预处理,探索茶树体胚诱导及增殖的适宜培养基和培养条件。结果表明:体胚诱导培养基:B5+6-BA 3.5~4 mg/L+IBA 1.5 mg/L^2 mg/L;MS+6-BA 2.0 mg/L^2.5 mg/L+IBA 0.2 mg/L为理想的继代增殖培养基。本研究对茶树体胚诱导及植株再生提供了一定的借鉴意义。In this resea rch, in order to obtain tea plant embryos, natural hybridization seeds of Fuding Dabai,Bixiangzao and Baihaozao were used as explants for tissue culture and plantlet regeneration. By optimizing the combination of different concentrations of plant growth regulator and the pretreatment of different medium and illumination, the suitable culture medium and conditions for the induction and proliferation of tea plant embryos were explored. The results showed that the embryo induction culture medium： B5＋6-BA 3.5~4 mg/L＋IBA 1.5 mg/L^2 mg/L; MS ＋6-BA 2.0mg/~2.5 mg/L ＋IBA 0.2 mg/L was the ideal subculture multiplication medium. This research provided a reference for the tea tree embryo induction and plant regeneration.

关 键 词：茶树 体胚 诱导 增殖 

分 类 号：S571.1[农业科学—茶叶生产加工]