我国首例输入性裂谷热病例病毒全基因组测序分析  

Whole-genome analysis of the virus from the first imported case of Rift Valley Fever in China

作　　者：潘阳[1] 崔淑娟[1] 陈丽娟[1] 吕燕宁[1] 卢桂兰[1] 孙玉兰[1] 李洁[1] 窦相峰[1] 李锡太[1] 黎新宇[1] 王全意[1] PAN Yang CUI Shu-juan CHEN Li-juan LYU Yan-ning L U Gui-lan SUN Yu-lan LI lie DOU Xiang- feng LI Xi-tai Xin-yu WANG Quan-yi(Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Diseases Prevention and Control （Beijing Center for Preventive Medical Research）, Beijing 100013, China)

机构地区：[1]北京市疾病预防控制中心(北京市预防医学研究中心)传染病地方病控制所,北京100013

出　　处：《国际病毒学杂志》2017年第1期1-4,共4页International Journal of Virology

基　　金：首都卫生发展科研专项项目（2014-1-1011）;北京市自然科学基金（7152075）

摘　　要：目的对我国首例输入性裂谷热病例病毒进行全基因组测定，分析其进化来源及潜在变异。方法提取样本核酸，非特异性反转录扩增病毒基因组RNA，使用Ion Torrent二代测序仪进行病毒全基因组测定。对获得的基因组数据进行序列拼接、比对、进化树构建和关键位点分析。结果通过测定获得了病毒全基因组11979nt，该测定病毒属E基因分支，序列与先前南非分离株Kakamas相似度最高（〉98％）。病毒Gn蛋白c端信号肽区存在1个氨基酸突变。结论本研究分析测定的裂谷热病毒全基因组与目前非洲流行株高度相似，病毒基因特征未出现明显变异。Objective To analyze the whole genome of rift valley fever virus （RVFV） derived from the first imported case of rift valley fever in China. Methods The genomic RNA was extracted from blood and saliva sample, and was reverse-transcripted into eDNA. Whole-genome sequencing by second generation sequencing （NGS） was achieved by using Ion Torrent PGM. Results The 11 979nt genome of RVFV was obtained by NGS. Phylogenetic analysis showed that the virus belonged to Clade E, and was highly homologous compared with Kakamas strains in South Africa. No significant effective mutations of amino acids were observed, except for M1V mutation in C-terminal signal region of Gn protein. Conclusions The RVFV virus analyzed in this study showed a very similar genomic feature compared with circulating isolates in African recently.

关 键 词：裂谷热病毒 全基因组测定 二代测序 基因变异 进化分析 

分 类 号：R373[医药卫生—病原生物学]