乳腺癌中Wnt信号通路成分NKD1、β-catenin和Cyclin D1蛋白的表达及意义  

Clinical significance of Wnt signal pathway member protein NKD1,β-catenin and Cyclin D1 expression in breast carcinoma

作　　者：邹娜 金波 王红龙 刘天卿[2] 

机构地区：[1]大连市妇女儿童医疗中心病理科,辽宁大连116037 [2]大连市友谊医院病理科,辽宁大连116001

出　　处：《大连医科大学学报》2017年第1期19-22,40,共5页Journal of Dalian Medical University

摘　　要：目的探讨NKD1、β-catenin和Cyclin D1在乳腺癌中的表达及意义。方法选取2010年至2016年大连市妇女儿童医疗中心和大连市友谊医院病理科30例正常乳腺组织和124例乳腺浸润性癌非特殊型标本及临床资料。采用免疫组化法检测标本中NKD1、β-catenin和Cyclin D1蛋白的表达,并分析其与临床病理参数之间的关系。结果在乳腺癌组织中NKD1阳性表达率为30.65%;β-catenin异常表达率为72.58%;Cyclin D1阳性表达率为54.03%,与正常乳腺组织比较,差异均有显著性意义(P均<0.05)。NKD1和β-catenin表达与乳腺癌组织学分级、p TNM分期及淋巴结转移有关,Cyclin D1表达与乳腺癌p TNM分期及淋巴结转移亦有关,且NKD1表达缺失还与ER状态相关,差异均有统计学意义(P均<0.05)。NKD1与β-catenin和Cyclin D1表达呈正相关(r分别为0.454,0.332,P均<0.05)。结论 NKD1、β-catenin和Cyclin D1蛋白表达与乳腺癌侵袭和转移密切相关,联合检测3种蛋白有助于判断乳腺癌生物学行为和评估预后。Objective To investigate the clinical significance of NKD1, β -catenin, Cyclin D1 protein expression in breast carcinoma. Methods Thirty normal breast tissues and 124 non - specific invasive breast cancer tissues were collected from Dalian Municipal Woman and Children＇ s Medical Center and Dalian Friendship Hospital. Immunohisto- chemistry was used to detect the expressions of NKD1, β -catenin, Cyclin D1 protein. Spss17. 0 software was used for statistical analysis. Results In breast cancer tissues, the negative frequency of NKD1 expression was 69.35%, the abnormal expression frequency of β - catenin was 72.58% , and the positive frequency of Cyclin D1 expression was 54.03%. There were significant differences between normal breast tissures and breast cancer tissues. Down - regulation of NKD1 expression and abnormal expression of β - catenin were significantly correlated with histologic grades, pTNM stages and the lymph node metastasis of breast cancer （P 〈 0.05 ）. Up - regulation of Cyclin D1 was significantly correlated with pTNM stages and the lymph node metastasis of breast cancer （ P 〈 0.05 ）. NKD1 expression was positively correlated with β - catenin abnormal expression and Cyclin D1 expression （ r = 0. 454, r = 0. 332, P 〈 0.05 ）. Conclusions NKD1,β - catenin, Cycfin D1 protein could become indicators of prognosis in breast cancer.

关 键 词：乳腺癌 NKD1 Β-CATENIN CYCLIN D1 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]