响应曲面法优化驼血多肽酶解工艺研究  

Study on Hydrolysis Process of Polypeptides from Camel Blood Optimized with Response Surface Methodology

作　　者：刘永峰[1,2] 刘毅[1,2] 徐大泽 邸多隆[1,2] 

机构地区：[1]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃天然药物重点实验室,兰州730000 [2]中国科学院兰州化学物理研究所青岛研究发展中心/青岛市资源化学与新材料研究中心,青岛266100 [3]甘肃中医药大学,兰州730000

出　　处：《食品工业》2017年第3期152-156,共5页The Food Industry

基　　金：内蒙古自治区科技计划项目;阿拉善盟科技计划项目;阿左旗科技计划项目;中科院“西部之光”项目;甘肃省自然科学基金项目(1501RJZA011);国家高技术研究发展计划(863计划,SS2014AA022203);青岛市民生科技计划项目(14-2-3-51-NSH)

摘　　要：试验以驼血为原料,利用响应曲面法(RSM)对驼血多肽的制备工艺进行优化。以酶解产物中低聚肽所占比例为响应值,考察蛋白酶A的加入量、酶解时间和酶解温度3个因素对驼血多肽酶解工艺的影响。在单因素试验的基础上,选取试验因素与水平,根据中心组合(Box-Behnken)试验设计原理,采用三因素三水平的响应曲面法对各个因素的显著性和交互作用进行分析。结果表明:驼血多肽酶解的最佳工艺条件为:蛋白酶A的加入量2.47%,酶解时间3 h,酶解温度60℃,该条件下酶解液中低聚肽所占比例为98.51%。试验可为从驼血中分离制备驼血多肽提供技术支持。The preparation process of polypeptides from camel blood was optimized by response surface methodology using camelblood as raw material in this study. Using the ratio of oligopeptides in the hydrolysate as response value, the effect on hydrolysis process was studied through the investigation of amount of protease A, hydrolysis time and hydrolysis temperature. The one-factor-at-a-time experiments were carried out to figure out the effects of three parameters on the preparation of polypeptides from camel blood. According to these results, the experiment factors and its levels were selected. And significance and interaction of each factor were analyzed on the basis of a three-variable, three-level central composite design based on Box-Behnken model. From the above investigations and analyses, optimum process conditions were determined to be： amount of protease A of 2.47%, hydrolysis time of 3 h and temperature of 60 ℃. Under the optimum conditions, from the view of theoretical calculation, ratio of oligopeptides in the hydrolysates was 98.51%. The results would provide the technical support for the preparation of polypeptides from camel blood.

关 键 词：驼血 多肽 蛋白酶 响应曲面法 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]