蜜蜂肠道纳豆发酵菌株的分离与鉴定  

Isolation and Identification of Natto Fermentation Strains in Honeybee Guts

作　　者：徐艳[1] 陈芳[1] 严文东 王淼[1] 李腾 董坤[1] XU Yan CHEN Fang YAN Wen-dong WANG Miao LI Teng DONG Kun(Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201,China Animal Husbandry and Veterinary Bureau, Huize 654200, China)

机构地区：[1]云南农业大学东方蜜蜂研究所,云南昆明650201 [2]云南会泽县畜牧兽医局,云南会泽654200

出　　处：《蜜蜂杂志》2017年第4期11-13,共3页Journal of Bee

基　　金：云南省重点研发计划项目资助(2016BC014)

摘　　要：蜜蜂肠道中的细菌种类丰富,其中的芽孢杆菌是优势菌。对东方蜜蜂肠道、西方蜜蜂肠道、市售纳豆产品、纳豆发酵粉中细菌进行分离与纯化,获得6株芽孢杆菌,通过传统培养进行形态学分析及16S rDNA测序比对,结果表明:蜜蜂肠道中与纳豆产品中存在2种相似菌株,分别是解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和枯草芽孢杆菌(Bacillus subtilis),这2种菌都可用于纳豆的发酵生产。There are abundant bacterial species in honeybee guts, among them Bacillus sp. is dominant bacteria. 6 isolates were selected and identified from honeybee guts in Apis cerana and Apis mellifera, Natto products and powder. Isolates were analyzed by morphology and 16S rDNA sequences, we found Bacillus amyloliquefaciens and Bacillus subtilis were present in honeybee guts and Natto products. They could be used in fermentation of natto.

关 键 词：蜜蜂肠道 解淀粉芽孢杆菌 枯草芽孢杆菌 鉴定 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]