基于microcarrier 6构建正常免疫小鼠人胃癌移植模型  

Establishment of human gastric cancer model in normal immune mice based on microcarrier 6

作　　者：毕研贞[1] 孔令斌[2] 高鹏飞 王全义[2] 杨永红[2] 张小蓓[2] 樊增[4] 王全全[1] 黄炳成 杨峰[2] 张秋生[2] 王一波[2] 孙富强[2] Ye Hong 洪丰[2] 

机构地区：[1]山东大学医学院,济南市250000 [2]济宁医学院附属医院 [3]大理大学,云南省昆虫生物医药研发重点实验室,药用特种昆虫开发国家地方联合工程研究中心 [4]济南大学医学与生命科学学院 [5]山东省寄生虫病防治研究所 [6]University of Turku

出　　处：《中国肿瘤临床》2017年第5期199-203,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目(编号:81170395;81570556;81260676)资助~~

摘　　要：目的:基于新型3D微载体(microcarrier 6)复合人胃癌MKN45细胞接种于具有正常免疫功能的小鼠,以期建立新型人胃癌正常小鼠转移瘤模型。方法:将60只雄性C57BL/6小鼠按是否将MKN45细胞接种于支架材料随机分为2D组、对照组和3D组;体外构建三维肿瘤细胞培养模型,采用皮下接种法建立小鼠移植瘤模型,观察小鼠成瘤时间、成瘤率、病理学表现等。结果:2D组和对照组均未成瘤,3D组成瘤率达80%,成瘤时间早,移植瘤HE染色和免疫组织化学均符合人胃癌特点。结论:本实验成功基于microcarrier 6复合人胃癌MKN45细胞在正常免疫小鼠中建立人胃癌小鼠移植瘤模型,此模型可以更好地研究和进一步阐明胃癌在免疫功能正常机体中发生、发展机制,同时也为抗癌药物的研发提供了更有价值的动物模型。Objective：To establish a mouse model of gastric cancer by inoculating MKN45 cells into mice with normal immune function utilizing microcarrier technology. Methods：A total of 60 male C57BL/6 mice were randomly divided into three groups, namely, 2D, con-trol, and 3D groups, according to the coculture system of MKN45 and microcarrier. The mouse models of gastric carcinoma were estab-lished by hypodermic injection. The time of tumorigenesis, rate of tumor formation, and pathological features were observed in each group. Results：In the 3D group, the time of tumor formation was short, whereas the rate of tumor formation was high （80%）. No de-tectable tumor formations were observed in the 2D and control groups. HE and immunohistochemical staining of the transplantation tumor model showed evident characteristics of human gastric cancer. Conclusion：A human gastric cancer model in normal immune mice was successfully established. The onset and development mechanism of gastric cancer could be more effectively investigated in mice with normal immune function through this model. Moreover, a more valuable and new animal model for the research and devel-opment of anticancer drug was established.

关 键 词：胃癌 MICROCARRIER MKN45细胞 动物模型 

分 类 号：R735.2[医药卫生—肿瘤] R-332[医药卫生—临床医学]