皇菊水溶性色素树脂纯化工艺及其稳定性研究  

Study on resin purification process and stability of water-soluble pigments from chrysanthemum

作　　者：胡洁芳 洪艳平[1] 刘玉珍[2] 曾璟 万琴[2] 胡远诚 张敏[2] 

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330045 [2]江西工业贸易职业技术学院,江西南昌330038 [3]会昌县职业技术学校,江西赣州342600

出　　处：《粮油食品科技》2017年第2期44-50,共7页Science and Technology of Cereals,Oils and Foods

摘　　要：以热水浸提法得到的皇菊色素为研究对象,利用DM-28大孔树脂纯化皇菊色素,优化其静、动态纯化工艺条件,并对纯化后的皇菊色素进行稳定性分析。结果表明,静态吸附—解吸最佳条件为:吸附平衡时间3 h,样液浓度为色素原液被稀释12倍(即A390=0.361),样液pH 2.5,吸附温度30℃,在pH 3.0、50%乙醇溶液条件下进行解吸;动态吸附—解吸最适工艺条件为:上样浓度为原液稀释12倍(即A390=0.361),上样流速2.0 mL/min,以流速1.0 mL/min、80%乙醇溶液进行洗脱。纯化后色素色价为22.5,是纯化前的3.65倍。DM-28大孔树脂具有良好的吸附性能,皇菊色素相对来说比较稳定,对外界条件不敏感,可以考虑作为一种天然食品色素应用到食品工业中。The pigment extracted from chrysanthemum by hot water method was selected as the investigate object,and purified by DM-28 macroporous resin.Static-dynamic purification conditions of the water-soluble pigments of chrysanthemum were optimized.The stability of the pigment was analyzed.The results showed that the optimal static adsorption-desorption conditions were as follow：adsorption equilibrium time was 3 h,the concentration of sample was the fluid diluted 12 times from the original（namely A390=0.361）,sample pH was 2.5,adsorption temperature was 30℃,with pH 3.0,50%ethanol as eluent for desorption.The optimal dynamic adsorption conditions were：sample concentration was A390=0.361,the sample flow rate was 2.0 mL/min,eluted with the flow rate of 1.0 mL/min,80%ethanol.The color value of the purified pigment was 22.5,which was 3.65 times of the former.DM-28 macroporous resin show better comprehensive adsorption property.The pigment was of relatively better stability,could be used as a kind of natural food additive in the food industry.

关 键 词：皇菊 水溶性色素 大孔树脂 稳定性 

分 类 号：R284.2[医药卫生—中药学]