水体微生物污染指示菌在不同营养水平下的稳定性  

Stability analysis of microbial pollution indicators in water under different nutrient levels

作　　者：张杨[1,2] 贾乐华 吴仁人[2,4] 陈中颖[2,4] 李开明[2,4] 张一敏[1,5] 

机构地区：[1]武汉理工大学资源与环境工程学院,湖北武汉430070 [2]环境保护部华南环境科学研究所,广东广州510655 [3]华南理工大学生物科学与工程学院,广东广州510006 [4]广东省水与大气污染防治重点实验室,广东广州510655 [5]武汉科技大学资源与环境工程学院,湖北武汉430081

出　　处：《中国环境科学》2017年第3期1130-1136,共7页China Environmental Science

基　　金：国家自然科学基金项目(41303054);中央级公益性科研院所基本科研业务专项

摘　　要：微生物污染指示菌在水体中的稳定性直接影响到定量的准确性及应用的适应性.通过建立水环境模拟反应器,以实时荧光定量PCR方法探究鸡粪中大肠埃希氏菌(EC)、拟杆菌(GB)及鸡源特异性基因(CB)等指示微生物在不同营养水平的水环境的稳定性及衰减特征.结果表明:所选微生物衰减较好的符合一级动力学模型.对照组和含有营养物质实验体系中指示微生物的衰减系数k分别为EC(0.135,0.134;前者为对照组,下同),GB(0.415,0.457),CB(0.425,0.437),说明营养物质对EC、GB、CB的衰减未有显著影响.有氧水环境中,GB、CB呈现快速衰减趋势,EC则变化相对缓慢.不同营养水平下3种微生物的衰减具有显著正相关性(P<0.05),均可作为良好指示菌以评价水体的微生物污染程度,但特征各异.Sensitivity and specificity have been used to assess the performance of fecal indicator in microbial source tracking. However, it is also important to characterize the stability of fecal indicators in aquatic habitats. In this study, indicators decay microcosm in aerobic circumstance was deployed. Decay characteristics of Escherichia coli（EC）, Bacteroides（GB） and Chicken-specific marker（CB） in different nutrition condition was evaluated based on real-time quantitative PCR assay. The results indicated that decay rate of three indicators was fit to first-order kinetics. In the control group and experimental system that contain high nutrients levels indicates the attenuation coefficient k of EC（0.135 vs. 0.134, the former as the control group, below the same as above）, GB（0.415 vs. 0.457）, CB（0.425 vs. 0.437） respectively. The effect of nutrition level on the decay rate of EC, GB and CB was inconspicuous. EC decayed gradually when compared with the severe attenuation of BC and CB. Although significant mutual linear correlationship of different indicators was observed, different characteristics of each indicator related to decay rate should specially be taken into consideration for further application.

关 键 词：微生物污染源解析 指示微生物 衰减 营养 溶解氧 

分 类 号：X172[环境科学与工程—环境科学] X52