贲门腺癌中Sox17蛋白表达及其与β-catenin、cyclin D1蛋白相关性研究  

Expression of Sox17 protein and correlation with β-catenin and cyclin D1 protein in gastric cardia adenocarcinoma

作　　者：潘盼[1] 邝钢[2] 郭艳丽[2] 郭炜[2] 董稚明[2] 杨飞[1] 

机构地区：[1]山东大学附属济南市中心医院病理科,山东济南250013 [2]河北医科大学第四医院肿瘤研究所,河北石家庄050011

出　　处：《癌变．畸变．突变》2017年第2期106-110,120,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省自然科学基金(H2013206315);河北省医学科学研究重点课题计划项目(20130543)

摘　　要：目的:检测贲门腺癌中Sox17蛋白表达及其与β-catenin和cyclin D1蛋白的相关性,探讨三者的表达状态与贲门腺癌发生发展的关系。方法:应用免疫组织化学法检测78例贲门腺癌及相应癌旁非肿瘤组织中Sox17、β-catenin及cyclin D1蛋白的表达情况及其与临床病理指标的关系。结果:在贲门腺癌组织中,Sox17、β-catenin和cyclin D1的阳性表达率分别为47.4%(37/78)、83.3%(65/78)和70.5%(55/78);在癌旁非肿瘤组织中,三者的阳性表达率分别为69.2%(54/78)、62.8%(49/78)和44.9%(35/78),差异均有统计学意义(P<0.01)。Sox17蛋白的阳性表达率与β-catenin和cyclin D1的阳性表达率均呈负相关(r=-0.264,P<0.05;r=-0.287,P<0.05);β-catenin与cyclin D1的阳性表达率呈显著正相关(r=0.913,P<0.05)。Sox17在Ⅰ、Ⅱ期的阳性表达率明显高于Ⅲ、Ⅳ期,二者比较差异有统计学意义(P<0.05)。β-catenin、cyclin D1的阳性表达率与淋巴结转移状态有关(P<0.05)。结论:Sox17蛋白在贲门腺癌中低表达,其可能通过异常活化Wnt/β-catenin信号转导通路,上调β-catenin蛋白及其下游靶蛋白cyclin D1的表达水平参与了贲门腺癌的发生发展。OBJECTIVE：To investigate expression of Sox17,β-catenin and cyclin D1 proteins in gastric cardia adenocarcinoma（GCA）,and to establish their relationship with pathogenesis of GCA.METHODS： Immunohistochemistry method was applied to examine the expression of Sox17,β-catenin and cyclin D1 proteins in 78 samples of GCA and the corresponding adjacent non-cancerous tissues.RESULTS：In GCA tissues,the frequency of Sox17,β-catenin and cyclin D1 expression was 47.4% （37/78）,83.3% （65/78） and 70.5%（55/78）,respectively, which was significantly lower than that in corresponding adjacent non-cancerous tissues：69.2%（54/78）,62.8%（49/78） and 44.9%（35/78）,（P〈0.01）. The frequency of β-catenin ectopic expression was 83.3%（65/78） in GCA tissues which was significantly higher than that in corresponding adjacent non-cancerous tissues of 62.8%（49/78） （P〈0.01）. Sox17 expression was negatively correlated with ectopic expression ofβ-catenin and cyclin D1 （r=-0.264,P〈0.05;r=-0.287,P〈0.05）. Sox17 were significantly correlated with tumor stage （P=0.045）.β-catenin and cyclin D1 were significantly correlated with lymph node metastasis（P〈0.05）. CONCLUSION：Low level expression of Sox17 protein may play an important role in the development of GCA via aberrantly activating the canonical Wnt/β-catenin signaling pathway through influencing the expression ofβ-catenin and cyclin D1.

关 键 词：Sox17 Β-CATENIN CYCLIN D1 WNT信号转导通路 贲门腺癌 

分 类 号：R735.2[医药卫生—肿瘤]