固相萃取-高效液相色谱法测定食品中的靛蓝和亮蓝  

Determination of indigo and brilliant blue in different types of food products by high performance liquid chromatography with solid phase extraction

作　　者：李静娜[1] 黄常刚[1] 梁高道[1] 肖永华[1] 革丽亚[1] 龚洁[1] 贺栋梁[2] 

机构地区：[1]武汉市疾病预防控制中心,武汉430015 [2]南华大学公共卫生学院

出　　处：《卫生研究》2017年第2期318-323,共6页Journal of Hygiene Research

摘　　要：目的建立固相萃取-高效液相色谱法测定多种类食品中靛蓝、亮蓝的检测方法。方法采用固相萃取技术,用乙腈水溶液提取样品中色素并经WAX小柱净化,以甲醇-0.02 mol/L乙酸铵溶液作为流动相梯度洗脱,经Waters Symmetry C_(18)(5μm,4.6 mm×250 mm)色谱柱分离,运用高效液相色谱-二极管阵列检测器(HPLC-PDA)检测。结果该方法靛蓝、亮蓝在0.50~20.00μg/m L范围内有较好的线性关系,均r>0.999;靛蓝和亮蓝的方法检出限分别为0.04和0.02 mg/kg;样品加标平均回收率为81.8%~101.1%,相对标准偏差为2.1%~4.9%(n=6)。结论该方法具有较高的选择性和灵敏度,回收率和重现性良好,可用于GB 2760—2014食品分类系统中多种类食品中靛蓝、亮蓝含量的分析。Objective To develop and validate a solid phase extraction-high performance liquid chromatographic （ SPE-HPLC ） method for the simultaneous determination of indigo and brilliant blue in different types of food products. Methods The artificial colors in food products were extracted by acetonitrile/water and purified by WAX SPE cartridges, The separation was achieved using a Waters Symmetry C18（5 μm, 4.6 mm × 250 mm） column and a binary gradient mobile phase of methanol and 0.02mol/ L ammonium acetate solution, detected by HPLC-PDA. Results The validated analytical method showed that there was a good linearity in the range of 0.05 -20.00 μg/mL for both indigo and brilliant blue （ r 〉 0. 999）. The lowest detection limits of indigo and brilliant blue were 0.04 and 0.02 mg/kg , respectively. The average recoveries were among 81.8% - 101. 1% , with relative standard deviation（RSD） of 2. 1% -4.9% （n = 6） for both artificial colors. Conclusion The method has high selectivity, high sensitivity, good recovery and reproducibility. It is suitable to simultaneously monitor indigo and brilliant blue in several types of food products based on the food classification system of GB 2760-2014.

关 键 词：高效液相色谱 固相萃取 靛蓝 亮蓝 食品检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS202.3[医药卫生—公共卫生与预防医学]