草酸脱羧酶的修饰及其对草酸钙的吸附研究  

Research on the adsorption of the modified oxalate decarboxylase on calcium oxalate

作　　者：蔡杏华 巫佳 林日辉[2,3] 贺俊斌[1] 武怡辰 王心雨 

机构地区：[1]广西民族大学海洋与生物技术学院,广西高校微生物与植物资源利用重点实验室,广西南宁530006 [2]广西民族大学化学化工学院,广西高校化学与生物转化过程新技术重点实验室,广西南宁530006 [3]广西多糖材料与改性重点实验室培育基地,广西南宁530006

出　　处：《食品工业科技》2017年第7期111-116,共6页Science and Technology of Food Industry

基　　金：国家教育部留学回国人员科研启动基金项目(教外司留[2012]1707号);广西自然科学基金项目(2014GXNSFAA118045);广西高等教育机构人才小高地创新团队计划(GJR201147)资助;广西科学研究与技术开发项目(桂科合14125008-1-2);2015年广西研究生教育创新计划资助研究生科研创新项目(YCSZ2015129)

摘　　要：为了提高草酸脱羧酶(OXDC)的适用性及其对草酸钙的吸附性能,本实验采用乙二胺四乙酸二酐(EDTAD)对草酸脱羧酶进行了化学修饰,通过高效液相色谱-质谱联用仪(UPLC-MS)测定其修饰位点,并研究和对比了酶在修饰前后对草酸钙的吸附差异,采用动力学和热力学吸附模型对吸附实验的结果进行拟合分析。实验结果表明,EDTAD成功修饰在草酸脱羧酶上,修饰位点位于肽N端的α-氨基上;且修饰酶(EDTAD-OXDC)对草酸钙的吸附作用,在酶初始浓度为5 mg/m L,p H为7.0,温度为37℃的条件下,比原酶(OXDC)提高了53.37%。结论:经EDTAD修饰后的草酸脱羧酶,其稳定性和吸附性能均有所提高。In order to improve the applicability and adsorption property of oxalate decarboxylase （ OXDC ） on calcium oxalate. EDTAD was used to modify the OXDC, modified site was determined by UPLC- MS, and the adsorption of the enzymes on calcium oxalate（ CaOx）were studied and compared.The kinetics and thermodynamics adsorption models were used to fit and analysis the experimental data. Results showed that EDTAD was successfully grafted onto OXDC covalently as an absorption group,the modified sites were located in the α-NH2 of the peptide N side, and the adsorption property of the modified enzyme （EDTAD-OXDC） on calcium oxalate was 53.37% higher than that of OXDC in initial enzyme concentration of 5 mg/mL, pH7.0,temperature of 37 ℃.Conclusion：which inclicated the stability and adsorption properties of DXDC was improved by grafting with EDTAD.

关 键 词：草酸脱羧酶 乙二胺四乙酸二酐(EDTAD) 修饰 草酸钙结石 吸附 

分 类 号：TS201.3[轻工技术与工程—食品科学]