人参皂苷Rg1抑制C2C12细胞MuRF-1/Atrogin-1表达研究  

Research on Ginsenoside Rg1 Inhibits MuRF-1/Atrogin-1 Expression in C2C12 Cells

作　　者：李凤玉[1] 贾胜男[1] 马爽[1] 赵洪岩[1] 霍洪亮[1] 

机构地区：[1]东北师范大学生命科学学院,吉林长春130024

出　　处：《长春师范大学学报》2017年第2期65-70,共6页Journal of Changchun Normal University

基　　金：国家自然科学基金项目"雌激素降解工程菌的构建与代谢调控机制研究"(51478096);吉林省重点科技攻关项目"人参皂苷护眼液研发"(20140204059YY)

摘　　要：采用MTT、流式细胞术和western blot检测人参皂苷Rg1对C2C12细胞活力、细胞凋亡、泛素连接酶E3表达的影响,并进一步研究了Rg1发挥作用的相关机制。结果表明,Rg1抑制细胞中MuRF-1/Atrogin-1的表达,并激活AKT使FoxO_(1/3a)磷酸化,从而抑制MuRF-1/Atrogin-1表达,Rg1的作用能被PI3K抑制剂LY294002所抑制。以上结果说明:Rg1对C2C12细胞具有抗细胞凋亡和蛋白质降解的作用,可为人参皂苷Rg1在临床肌萎缩的治疗和预防提供新的思路和方法。The effects of ginsenoside Rg1 on cell viability,apoptosis,expression of ubiquitin ligase E3 in C2C12 cells were detected by MTT,flow cytometry and western blot,and the mechanism of Rg1’s action was further studied. The results showed that Rg1 inhibited the expression of MuRF-1 / Atrogin-1,and activated AKT to phosphorylate FoxO1 /3a,thus inhibiting the expression of MuRF-1 / Atrogin-1. The function of Rg1 can be inhibited by PI3 K inhibitor LY294002. These results indicate that Rg1 has anti-apoptotic and protein degradation effects on C2C12 cells,providing a new idea and method for ginsenoside Rg1 in the treatment,prevention and prognosis evaluation of clinical muscular atrophy.

关 键 词：RG1 肌萎缩 PI3K/AKT FOXO 

分 类 号：Q445[生物学—生理学]