EGFR在三阴性乳腺癌中的表达及放疗后表达变化的意义  

Expression of EGFR in Triple Negative Breast Cancer and the Significance of Expression Changes after Radiotherapy

作　　者：王璐[1] 林清[1] 陆春花[1] 张颖[1] 董晓庆[1] 石静[1] 贾朝阳[1] 

机构地区：[1]同济大学附属第十人民医院放疗科,上海200072

出　　处：《医学研究杂志》2017年第3期120-123,共4页Journal of Medical Research

摘　　要：目的探讨EGFR与三阴性乳腺癌患者临床与病理的关系及X射线对人三阴性乳腺癌细胞EGFR基因表达的影响。方法回顾性分析EGFR与88例三阴性乳腺癌患者临床和病理指标之间的关系,并用2.5、5和10 Gy X射线照射体外培养的人三阴性乳腺癌MDA-MB-231细胞和HCC1937细胞,采用荧光实时定量PCR技术检测两种细胞经照射后6、12、24和48h的EGFR mRNA表达水平,以未照射组为对照。结果 EGFR在三阴性乳腺癌的阳性表达率为67.0%(59/88),且与年龄、肿块直径、脉管侵犯、组织学分级、ki-67等临床及病理指标之间差异无统计学意义(P>0.05),但在淋巴结有转移及临床分期Ⅲ期组的表达率明显升高(P<0.05)。EGFR mRNA的表达在2.5、5和10Gy X射线照射后早期整体下降,但晚期呈上升趋势,大部分时间点差异有统计学意义(P<0.05)。结论 EGFR在三阴性乳腺癌中具有较高的表达率,且与淋巴结转移和临床分期有关,提示其可能与三阴性乳腺癌的预后不良相关。较大剂量X射线在早期可下调MDA-MB-231细胞和HCC1937细胞EGFRmRNA的表达,可能控制了肿瘤细胞的增殖和远处转移,提示放疗在三阴性乳腺癌的治疗中具有重要作用;但在晚期EGFRmRNA的表达有所回落甚至上升,可能与三阴性乳腺癌细胞易形成辐射抗性有关。Objective To investigate the relationship with the expression of EGFR and clinicopathological in triple negative breast cancer and to study the effects of X - rays radiation on the expression of EGFR gene in human triple negative breast cancer cells. Methods The expression of EGFR in 88 cases of triple negative breast cancer was summarized and the relationship with clinicopathological was studied. MDA - MB - 231cells and HCC1937 cells were exposed to different doses of X - rays （2.5 ,Sand 10 Gy） and the relative level of EGFR mRNA in the cells was detected by using real time - PCR at 6, 12, 24 and 48h after irradiation. The untreated MDA - MB -231 cells and HCC1937 cells were used as the control. Results （1）The expression of EGFR in triple negative breast cancer was 67.0% （59/ 88） and had no correlation with age, tumorsize, vascular invasion, histological grade, expression of Ki - 67 （ P 〉 0.05 ）, but it was much higher in lymph node metastasis group and clinical stage Ⅲ group, and the difference was statistically significant（ P 〈 0.05 ）. （2）The expression of EGFR mRNA trended to decrease after irradiated with 2.5,5and 10Gy in the earlier period and increased on the advanced stage with the statistical difference at the most time points（P 〈 0.05）. Conclusion The expression of EGFR in triple negative breast cancer was higher and significantly related with lymphnone metastasis and clinical stages,which indicates that it may be related to poor prognosis of triple negative breast cancer. Higher doses of X - rays may degrade the expression of EGFR mRNA in human MDA - MB - 231 cells and HCC1937 cells in the earlier period, then may control the proliferation and distant metastasis,which indicates that radiotheraphy may play an important role in triple negative breast cancer. But X - rays also may upgrade the expression of EGFR mRNA on the advanced stage, which might be correlated with the radiation resistance of the triple negative breast cancer ceils.

关 键 词：三阴性乳腺癌 EGFR X射线 照射 

分 类 号：R73[医药卫生—肿瘤]